高迁移率族蛋白1在胃癌中的表达及其与相关临床病理特征的关系

吴震+欧阳晓晖+苏秀兰

[摘要] 目的 研究高遷移率族蛋白1（HMGB1）在胃癌组织的表达，以及其与相关胃癌临床病理特征的关系。方法 选取内蒙古医科大学第一附属医院2012年3月～2013年9月术后病理明确诊断的104例胃癌及其癌旁组织标本，应用免疫组织化学法检测标本HMGB1蛋白的表达，分析HMGB1在胃癌组织和癌旁组织的差异及其与胃癌临床病理特征关系。检测HMGB1 mRNA在胃癌细胞系SGC-7901、N87、MKN45及正常胃黏膜细胞系GES-1中的表达。 结果 免疫组化显示：HMGB1阳性颗粒不仅位于细胞核上，还位于细胞质中；胃癌组织中的表达水平高于胃癌的癌旁组织（P < 0.01）。QPCR均显示：HMGB1 mRNA在正常胃黏膜细胞中的表达水平低于低分化胃癌细胞（P < 0.01）。 结论 HMGB1在胃癌组织中表达较高，特别是低分化胃癌，推测可能参与胃癌发生和发展，但在胃癌浸润转移中的临床意义需要扩大样本量进行进一步研究。

[关键词] 高迁移率族蛋白1；胃癌；免疫组织化学；QPCR

[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-7210（2017）03（b）-0009-04

Expression of HMGB1 in gastric cancer and its relationship with clinical pathological characteristics

WU Zhen1，2 OUYANG Xiaohui1 SU Xiulan1

1.Clinical Medical Research Center， Affiliated Hospital of Inner Mongolia Medical University， Inner Mongolia Autonomous Region， Huhhot 010050， China； 2.Graduate School of Medical Sciences， Baotou Medical College of Inner Mongolia University of Science and Technology， Inner Mongolia Autonomous Region， Baotou 014010， China

[Abstract] Objective To study the expression of HMGB1 in gastric carcinoma and the relationship between the expression and clinicopathological features of gastric cancer. Methods From March 2012 to September 2013， in the First Affiliated Hospital of Inner Mongolia Medical University， 104 samples of postoperative pathological diagnosis gastric cancer and their adjacent tissues were selected. Immunohistochemical method was used to detected HMGB1 protein expression， differences of HMGB1 in gastric cancer tissues and adjacent tissues， and its relationship with clinical pathological features of gastric carcinoma were analyzed， the expression of mRNA HMGB1 in gastric cancer cell line SGC-7901， N87， MKN45 and normal gastric mucosal cell line GES-1 were detected. Results Immunohistochemical staining showed that HMGB1 positive particles not only were located in the nucleus， but also in the cytoplasm， and its expression level in gastric cancer tissue was higher than that in gastric cancer adjacent tissue （P < 0.01）. QPCR showed that HMGB1 mRNA in normal gastric mucosal cells was lower than that in poorly differentiated gastric carcinoma cells （P < 0.01）. Conclusion HMGB1 is highly expressed in gastric cancer tissues， especially in poorly differentiated gastric carcinoma and speculated that it may participate in the process of the occurrence and development of gastric cancer， but in gastric cancer invasion and metastasis and its clinical significance need to expand the sample size for further study.