mdig基因在人胰腺癌中的表达及其促癌作用

罗和生+占婷+田霞+黄晓东

[摘要] 目的 研究mdig基因在胰腺癌细胞中的表达情况，探讨mdig与胰腺癌发生发展的关系及其作用的可能机制。 方法 采用实时荧光定量聚合酶链反应（Q-PCR）和Western blot方法检测mdig在胰腺癌细胞PANC1和MIAPaca2与正常胰腺导管上皮细胞HPDE6c7中表达的差异。将转染mdigsi RNA质粒的胰腺癌细胞PANC1和MIAPaca2设为实验组，将转染空载质粒的胰腺癌细胞PANC1和MIAPaca2设为对照组，用CCK8法和流式测凋亡法分别检测两组细胞的增殖和凋亡情况；用Western blot检测两组细胞的Sox2基因表达情况。 结果 与正常胰腺导管上皮细胞HPDE6c7相比，mdig基因在胰腺癌细胞PANC1和MIAPaca2中表达均上调（P < 0.05）。与对照组相比，实验组细胞的增殖率下降（P < 0.05），凋亡率增高（P < 0.05），Sox2的表达下调（P < 0.05）。 结论 mdig在胰腺癌细胞中表达升高，并可能通过介导原癌基因Sox2发挥促癌作用。

[关键词] 胰腺癌；矿物粉尘诱导基因；增殖；凋亡

[中图分类号] R735 [文献标识码] A [文章编号] 1673-7210（2017）03（b）-0012-04

Expression of mdig gene in human pancreatic carcinoma and its role in promoting cancer

LUO Hesheng1 ZHAN Ting1 TIAN Xia HUANG Xiaodong2

1.Department of Gastroenterology， People's Hospitial of Wuhan University， Hubei Province， Wuhan 430060， China； 2. Department of Gastroenterology， Tongren Hospital of Wuhan Univerity， Hubei Province， Wuhan 430060， China

[Abstract] Objective To study the expression of mdig in pancreatic cancer cells， in order to explore the relationship between mdig and the development of pancreatic cancer and its possible mechanism. Methods The expression of mdig in pancreatic cancer cells and normal pancreatic ductal epithelial cells were detected by Q-PCR and Western blot. The pancreatic cancer cells PANC1 and MIAPaca2 transfected with mdigsi RNA plasmid were designated as the experimental group， and the pancreatic cancer cells PANC1 and MIAPaca2 transfected with empty plasmid were designated as control group， the proliferation of two groups of cells was detected by CCK8， the apoptosis of two groups of cells was detected by flow cytometry， the expression of Sox2 in two groups of cells was detected Western blot. Results Compared with normal pancreatic ductal epithelial cells HPDE6c7， the expression of mdigin pancreatic cancer cells PANC1 and MIAPaca2 was up-regulatede. Compared with the control group， the proliferation rate of the experimental group was decreased （P < 0.05）， the apoptosis rate was increased （P < 0.05）， and the expression of Sox2 was down regulated （P < 0.05）. Conclusion The expression of mdig in pancreatic cancer cells increases， and mdig may regulate the expression of Sox2 to promoting the occurrence of pancreatic cancer.

[Key words] Pancreatic cancer； Mdig； Proliferation； Apoptosis

近年來，胰腺癌的发生率和死亡率逐渐升高，已成为癌症致死的第4位病因[1]，它的侵袭性生长和早期转移扩散使其成为最具侵袭性的肿瘤之一，约40%患者在确诊时已经发生局部或远处转移，失去根治性手术的机会[2]。随着研究的深入发现，肿瘤的发生往往是多种基因共同作用的结果[3-4]因此可以通过研究与胰腺癌发病密切相关的基因，从而在分子水平上寻找其中的关键基因并开发相应的靶向治疗。矿物粉尘诱导基因（mineral dust induced gene，mdig）于2005年作为一种新肺癌基因被发现[5]，随后被证实与mina53基因是同一种基因，而mina53基因是原癌基因c-myc的靶基因[6]。越来越多的研究证实mdig基因是一种重要的肿瘤相关基因，与某些肿瘤的发生与发展密切相关，并且已经有报道发现mdig在人类结肠癌[7]，食管鳞状细胞癌[8]、肝细胞癌[9]、胆管细胞癌[10]表达均升高。然而，目前只有极少数关于mdig基因在胰腺癌组织中的表达情况的报道，而且其表达情况的定量研究及其与胰腺癌发生发展的关系还未见报道。本研究拟探讨mdig基因在胰腺癌细胞中表达状况及其促癌作用并探讨其发挥作用的可能机制。