唑来膦酸对去势大鼠骨折后骨痂和骨微结构的影响

陆永江+刘华

[摘要] 目的 探讨唑来膦酸对去势大鼠骨折后骨痂和骨微结构的影响。 方法 将45只SD雌性大鼠分为骨质疏松组（30只）和正常对照组（15只），骨质疏松组雌性大鼠被切除双侧卵巢制作骨质疏松动物模型，正常对照组大鼠仅给予假手术；骨质疏松模型建模成功后，将骨质疏松组30只大鼠再次随机分为生理盐水组（15只）和唑来膦酸组（15只）；对两组大鼠制作股骨骨折模型，并分别给予生理盐水和唑来膦酸进行干预；在药物干预4周后，比较生理盐水组和唑来膦酸组骨质疏松大鼠股骨骨痂和骨微结构情况。 结果 卵巢切除术后8周，正常对照组和骨质疏松组大鼠股骨的骨密度分别为（0.197±0.028）g/cm2和（0.128±0.037）g/cm2，差异有统计学意义（P < 0.05）；在药物干预4周后，两组骨质疏松大鼠股骨均有骨痂形成，而唑来膦酸组大鼠骨痂明显大于生理盐水组，且骨折线较为模糊；与生理盐水组大鼠比较，唑来膦酸组大鼠骨小梁间隙较小，排列也较为整齐致密，更接近正常对照组大鼠。 结论 唑来膦酸可促进骨质疏松大鼠骨折后骨痂形成，并提高其骨密度。

[关键词] 唑来膦酸；骨质疏松；股骨骨折；骨痂；骨微结构

[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210（2014）02（c）-0011-04

Effects of Zoledronic Acid on callus and bone microstructure after fracture in osteoporosis rats

LU Yongjiang LIU Hua▲

Department of Orthopedics， the 474th Hospital of People's Liberation Army， Xinjiang Uygur Autonomous Region， Urumqi 830013， China

[Abstract] Objective To investigate the effects of Zoledronic Acid on callus and bone microstructure after fracture in osteoporosis rats. Methods 45 SD female rats were divided into osteoporosis group （30 cases） and control group （15 cases）， the female rats osteoporosis group were ovariectomized to make model of osteoporosis， the rats of normal control were only given sham-operated； after the model of osteoporosis was made successfully， the 30 rats of osteoporosis group were again randomly divided into saline group （15 cases） and Zoledronic Acid group （15 cases）； all rats of osteoporosis group were made femur fracture model， and been given saline and Zoledronic Acid to treatment； 4 weeks after drug intervention， the callus and bone microstructure situation of rats were observed and compared. Results At 8 weeks after ovariectomy， the BMD of femur in SD rats of osteoporosis group and control group were （0.197±0.028） g/cm2 and （0.128±0.037） g/cm2 kespectively， the difference was significant （P < 0.05）； after 4 weeks of drug intervention， all rats of two groups had osteoporosis femur bone callus formation， and callus in rats of Zoledronic Acid group was significantly greater than that of the saline group， and the fracture line was more blurred； compared with the rats of saline group， the trabecular bone gap in rats of Zoledronic Acid group was more smaller， compact and tidy arrangement was relatively closer to the normal control group. Conclusion Zoledronic Acid can promote the rat osteoporotic bone fracture callus formation and increase bone density.

[Key words] Zoledronic Acid； Osteoporosis； Femoral fractures； Callus； Bone microstructure