APP下载

入侵安徽省草地贪夜蛾的遗传分析

2019-11-27徐丽娜胡本进苏贤岩

植物保护 2019年5期
关键词:分子鉴定草地贪夜蛾

徐丽娜 胡本进 苏贤岩

摘要 2019年5月17日在安徽省黄山市黄山区发现草地贪夜蛾为害春玉米,首次证实草地贪夜蛾入侵安徽。本研究采集安徽省7市13县(区)草地贪夜蛾样本共167份,利用线粒体细胞色素C氧化酶亚基Ⅰ基因(cytochrome c oxidase subunit Ⅰ, COⅠ),以及位于Z染色体上的磷酸甘油醛异构酶基因(triosephosphate isomerase, Tpi)两个分子标记对样品的群体遗传特征进行分析。基于线粒体COⅠ基因分析结果显示,安徽省草地贪夜蛾样本中86.23%是水稻型,13.77%是玉米型,而且安徽地区样品COⅠ序列特征与美国佛罗里达州种群高度一致。针对水稻型和玉米型的Tpi基因序列10 个差异单倍型位点进行分析,发现安徽草地贪夜蛾样品Tpi基因的第3和4位差异单倍型位点有3种不同类型。其中118个样本为玉米型(AT),20个样本为水稻型(GA),29个样本为杂合型(AT/GA),但根据差异单倍型位点的数量判断,所有样品仍属于玉米型。草地贪夜蛾在原产地的多种寄主,如玉米、水稻、大豆、棉花等,在安徽省均有种植,该虫对安徽省粮食生产构成巨大安全隐患。

关键词 草地贪夜蛾; 分子鉴定; 玉米型; 水稻型

中图分类号: S 433.4  文献标识码: A  DOI: 10.16688/j.zwbh.2019348

Abstract On 17th May, 2019, the fall armyworm, Spodoptera frugiperda (J.E.Smith), was observed on damaged corn plants in Huangshan district, which meant the invasion of the S.frugiperda was confirmed in Anhui province for the first time. In this study, a total of 167 samples of S.frugiperda were collected from 7 cities in Anhui province. The population genetic characteristics of the collected samples were analyzed by using mitochondrial cytochrome C oxidase subunit Ⅰ gene (COⅠ), and glyceraldehyde phosphate isomerase gene (Tpi) located on the Z chromosome. Based on analysis of COⅠgene, the 86.23% of S.frugiperda in Anhui province was identified as rice strain and 13.77% was corn strain, respectively. In addition, the COⅠsequence characteristics of S.frugiperda in Anhui province was highly consistent with that of the population in Florida, USA.Based on the 10 different haplotype loci of Tpi gene sequences, which were used to define the subpopulations of S.frugiperda, three different types were exited at the haplotype loci 3 and 4 of Tpi gene. Among them, 118 samples were corn strain haplotype (AT), 20 samples were rice strain haplotype (GA), and 29 samples were hybrid type (AT/GA). However, all the collected samples were still belonged to corn strain, according to the number of different haplotype. Various hosts of S.frugiperda in the origin place, such as corn, rice, soybean and cotton, are cultivated in Anhui province. Therefore, this insect poses a huge potential security hazard to the grain production of Anhui province.

Key words Spodoptera frugiperda; molecular identification; corn strain; rice strain

2019年1月11日,在我國云南省普洱市江城县首次发现草地贪夜蛾Spodoptera frugiperda (J.E.Smith)为害玉米[1],此后该虫在我国的为害范围迅速扩大。2019年5月17日,在黄山市黄山区仙源镇洋家村首次发现草地贪夜蛾在安徽省为害玉米,至今安徽全省已有12市57区(县)发现该虫为害,发生面积达到1.1万 hm2。

草地贪夜蛾幼虫食量大、食性杂,可为害76科353种寄主植物[2],其中玉米、高粱、水稻、小麦、大豆、棉花、牧草均是其在原产地美洲的主要寄主。在其原产地美洲,草地贪夜蛾有玉米型和水稻型两种生物型,两种生物型在寄主偏好、雌性信息素成分、交配行为等生物学及生理学特征方面具有较为明显的差异[3-4],该虫2016年侵入非洲,目前两种亚型均已在非洲定殖为害[5]。虽然安徽省目前仅在玉米田发现草地贪夜蛾为害,但入侵广东省的草地贪夜蛾种群中已发现水稻型个体存在[6]。

[6] LIU Huan,LAN Tianming,FANG Dongming,et al.Chromosome level draft genomes of the fall armyworm,Spodoptera frugiperda (Lepidoptera:Noctuidae),an alien invasive pest in China [J/OL].bioRxiv,2019,doi:http:∥dx.doi.org/10.1101/671560.T.

[7] MEAGHER R L,GALLOMEAGHER M.Identifying host strains of fall armyworm (Lepidoptera:Noctuidae) in Florida using mitochondrial markers [J].Florida Entomologist,2003,86(4):450-456.

[8] NAGOSHI R N,MUR U 'A M G,HAYROE M,et al.Genetic characterization of fall armyworm (Lepidoptera:Noctuidae) host strains in Argentina [J].Journal of Economic Entomology,2012,105(2):418-428.

[9] NAGOSHI R N.The fall armyworm triose phosphate isomerase (Tpi) gene as a marker of strain identity and interstrain mating [J].Annals of the Entomological Society of America,2010,103(2):283-292.

[10] NAGOSHI R N,KOFFI D,AGBOKA K,et al.Comparative molecular analyses of invasive fall armyworm in Togo reveal strong similarities to populations from the eastern United States and the Greater Antilles [J/OL].PLoS ONE,2017,12(7):e0181982.

[11] KALLESHWARASWAMY C M,ASOKAN R,MAHADEVASWAMY H M.First record of invasive fall armyworm,Spodoptera frugiperda (J.E.Smith) (Lepidoptera:Noctuidae) on rice (Oryza sativa) from India [J].Journal of Entomology and Zoology Studies,2019,7(3):332-337.

[12] JUREZ M L,MUR U 'A M G,GARCA M G,et al.Host association of Spodoptera frugiperda (Lepidoptera:Noctuidae) corn and rice strains in Argentina,Brazil,and Paraguay[J].Journal of Economic Entomology,2012,105(2):573-582.

[13] OTIM M H,TAY W T,WALSH T K,et al.Detection of sisterspecies in invasive populations of the fall armyworm Spodoptera frugiperda (Lepidoptera:Noctuidae) from Uganda [J/OL].PLoS ONE,2018,13(4):e0194571.

[14] COCK M J,BESEH P K,BUDDIE A G,et al.Molecular methods to detect Spodoptera frugiperda in Ghana,and implications for monitoring the spread of invasive species in developing countries [J/OL].Scientific Reports,2017,7(1):4103.

[15] NAGOSHI R N,GOERGEN G,TOUNOU K A,et al.Analysis of strain distribution,migratory potential,and invasion history of fall armyworm populations in northern SubSaharan Africa[J/OL].Scientific Reports,2018,8(1):3710.

[16] 張磊,靳明辉,张丹丹,等.入侵云南草地贪夜蛾的分子鉴定[J].植物保护,2019,45(2):19-24.

[17] JING Daping,GUO Jingfei,JIANG Yuying,et al.Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfield using molecular techniques[J].Insect Science,2019,https:∥doi.org/10.1111/1744-7917.12700.

[18] 张磊,柳贝,姜玉英,等.中国不同地区草地贪夜蛾种群生物型分子特征分析[J].植物保护,2019,45(4):20-27.

[19] 郭井菲,赵建周,何康来,等.警惕危险性害虫草地贪夜蛾入侵中国[J].植物保护,2018,44(6):1-10.

[20] SIDANA J,SINGH B,SHARMA O.Occurrence of the new invasive pest,fall armyworm,Spodoptera frugiperda (J E Smith) (Lepidoptera:Noctuidae),in the maize fields of Karnataka,India [J].Current Science,2018,115(4):621.

[21] KALLESHWARASWAMY C M,ASOKAN R,SWAMY H M,et al.First report of the fall armyworm,Spodoptera frugiperda (J.E.Smith) (Lepidoptera:Noctuidae),an alien invasive pest on maize in India[J].Pest Management in Horticultural Ecosystems,2018,24(1):23-29.

[22] IPPC.First detection of fall army worm on the border of Thailand [EB/OL].IPPC Official Pest Report,No.THA03/1 .FAO:Rome,Italy.2018,https:∥www.ippc.int/.

[23] IPPC.First detection report of the fall armyworm Spodoptera frugiperda (Lepidoptera:Noctuidae) on maize in Myanmar[EB/OL].IPPC Official Pest Report,No.MMR19/2.FAO:Rome,Italy.2019,https:∥www.ippc.int/.

[24] IPPC.Report of first detection of fall armyworm (FAW) in Republic of Korea [EB/OL].IPPC Official Pest Report,No.KOR08/2.FAO:Rome,Italy.2019,https:∥www.ippc.int/.

[25] IPPC.Report of first detection of Spodoptera frugiperdafall armyworm (FAW) in Egypt [EB/OL].IPPC Official Pest Report,No.EGY01/1.FAO:Rome,Italy.2019,https:∥www.ippc.int/.

[26] IPPC.Report of first detection of Spodoptera frugiperdafall armyworm (FAW) in Japan [EB/OL].IPPC Official Pest Report,No.JPN08/6.FAO:Rome,Italy.2019,https:∥www.ippc.int/.

[27] FAO.Briefing note on FAO actions on fall armyworm[R].Rome,Italy.2019,http:∥www.fao.org/3/abs183e.pdf.

[28] PASHLEY D P,MARTIN J A.Reproductive incompatibility between host strains of the fall armyworm (Lepidoptera:Noctuidae)[J].Annals of the Entomological Society of America,1987,80(6):731-733.

[29] DUMAS P,LEGEAI F,LEMAITRE C,et al.Spodoptera frugiperda (Lepidoptera:Noctuidae) hostplant variants:two host strains or two distinct species?[J].Genetica,2015,143(3):305-316.

[30] NAGOSHI R N,DHANANI I,ASOKAN R,et al.Genetic characterization of fall armyworm infesting South Africa and India indicate recent introduction from a common source population [J/OL].PLoS ONE,2019,14(5):e0217755.

(責任编辑: 田 喆)

猜你喜欢

分子鉴定草地贪夜蛾
草地贪夜蛾对圣多美和普林西比玉米的危害和防治
新物种入侵—草地贪夜蛾的防治方法
永靖县草地贪夜蛾监测防控技术策略
不同农药防治甜玉米草地贪夜蛾的药效对比试验
草地贪夜蛾对山东省玉米的危害风险及其监测防控研究进展
安徽省草地贪夜蛾入侵概况及其防治措施
徐州四种市售食用菌的分子鉴定
ITS2序列分析在白花蛇舌草鉴定中的应用研究
艾草白粉病的病原菌鉴定