Effects of sepsis on hippocampal volume and memory function

Miao Yuan, Ding-yi Yan, Fang-shi Xu, Yi-di Zhao, Yang Zhou, Long-fei Pan

1 Emergency Department, the Second Aff iliated Hospital of Xi’an Jiaotong University, Xi’an, China

2 Department of Cardiology, Xi’an No. 3 Hospital, Xi’an, China

KEYWORDS: Hippocampus; Magnetic resonance imaging; Memory; Sepsis-associated encephalopathy

INTRODUCTION

Sepsis is a life-threatening, multiple-organ dysfunction condition caused by an infection that induces immune dysfunction in the host.[1]It is an important medical and health problem that yearly affects thousands of patients worldwide. Sepsis-associated encephalopathy(SAE) is a common complication of sepsis. SAE refers to diffuse brain dysfunction. The incidence of SAE is 8%-70%, and it is also one of the most common brain disorders in the intensive care unit.[2,3]So far, there are no clear diagnostic criteria for SAE. SAE diagnosis excludes direct infection of the central nervous system,head trauma, fat embolism, adverse drug reactions, other factors that affect consciousness, and diffuse cerebral dysfunction caused by the systemic inflammatory response.[4,5]A multicenter study found that 307 (23.0%)of 1,333 patients with severe sepsis had severely altered mental states.[2]However, sensitive diagnostic tools,such as electrophysiological tests for brain function,revealed that almost all sepsis patients possessed mental abnormalities.[6-8]Brain dysfunction is often overlooked because sepsis patients are not diagnosed with SAE during hospitalization.

SAE is a disorder with complex and unclear pathogenesis.[9]Cerebral microcirculation is one important pathophysiological symptom of SAE.[10]In sepsis patients with impaired brain autoregulation, the cerebral vessel responses to carbon dioxide concentration and extracellular pH become slow.[11]When systemic blood pressure drops sharply, inadequate cerebral perfusion will likely occur,leading to neurological dysfunction. Dysfunction of brain autoregulation leads to an inability to respond to changes in mean arterial pressure.[6,10,12,13]Related studies have shown that neuronal damage after cerebral ischemia is regionselective, and the vulnerabilities of neurons in sensitive areas are also different.[14]The hippocampus and neocortex are prone to damage after cerebral ischemia. Neurons in the CA1 area of the hippocampus are especially sensitive to transient ischemia, whereas apoptosis in the CA3 area is known as selective vulnerability. Sepsis patients have a higher risk of non-Alzheimer’s dementia compared with the general population.[15,16]At present, most clinical diagnoses of SAE are based only on clinical manifestations without electrophysiological or biochemical tests. As a result,many sepsis patients with hidden neurological dysfunction do not undergo comprehensive neuropsychological tests because they do not have signif icant psychiatric disorders in hospitals. The nervous system function in such patients is not clear; thus, most sepsis patients don’t receive corresponding rehabilitation treatment.

This study aimed to investigate whether sepsis causes loss of brain volume and cognitive function.We used magnetic resonance imaging (MRI) scans to evaluate the nervous system integrity of sepsis patients and healthy controls. We focused on the hippocampus,thalamus, caudate, putamen, pallidum, amygdala, and ventral diencephalon because these areas are considered as the most appropriate regions for analysis throughout the brain. The CA1 area of the hippocampus was also analyzed because CA1 is particularly sensitive to ischemia and hypoxia.[14]Neuropsychological tests were used to assess the neurological function of patients.Particular emphasis was placed on the cognitive and executive functions, which are susceptible to be inf luenced by ischemia and hypoxia.[17]

METHODS

Study population

This study included 20 sepsis patients who were not diagnosed clinically with SAE but had abnormal electroencephalograms (EEGs). These patients were admitted to the emergency department of the Second Affiliated Hospital of Xi’an Jiaotong University from June 2015 to November 2018, and the clinical data were collected and analyzed.

The patients were diagnosed with sepsis, which was def ined in accordance with the criteria.[1]All patients had no change of mental state during hospitalization, but their EEGs revealed abnormalities, including background abnormalities, changes in cycles and rhythms, and epileptiform activity. Background abnormality was characterized by slow background activity, including diffuse slow waves, persistent θ waves, and slow delta waves or burst suppression, with or without autonomous background variation. The periodic and rhythmic changes were manifested as three-phase waves, periodic epileptic rhythms, intermittent rhythmic δ activities in the frontal region, pseudoperiodic epileptic discharges on one side or epileptic discharges on both sides, and abnormal seizure periods. All sepsis patients completed Acute Physiology and Chronic Health Evaluation II (APACHE II)[18]scoring (score 0 to 71) on admission. After admission, patients received empirical anti-infection treatment. For patients with positive blood cultures in the later stage, the anti-infection regimen was adjusted according to the blood culture results. All patients with hemodynamic abnormalities received active fluid resuscitation, and crystalloid fluid was preferred. All patients underwent an MRI examination within one month after hospital discharge. The control group was composed of 20 healthy people (from the Physical Examination Center of the Second Aff iliated Hospital of Xi’an Jiaotong University);their sex, age, education level, hypertension, coronary heart disease (CHD), diabetes mellitus (DM), and other basic information were matched with those of the sepsis group.All participants were required to have no previous history of neurological diseases so as not to affect the results of this study. All participants underwent neuropsychological tests within one month after enrolling in the study. Table 1 summarizes the basic demographic information and clinical characteristics of the two groups.

Neuropsychological assessments

Neuropsychological assessments were performed in all sepsis patients and healthy controls. All sepsis patients completed the test within one month after discharge, and participants in the control group completed the test within one month after joining the study. The assessments included the following cognitive functions: (1) general intelligence test using the Wechsler Adult Intelligence Scale-III (Chinese translation version);[19](2) visual evaluation using the Hooper Visual Organization Test;[20](3) memory function using the California Verbal Learning Test-II (CVLT-II);[21](4) attention using the Stroop Test (ST);[22](5) executive function using the Wisconsin Card Sorting Test;[23](6)working memory using the Paced Auditory Serial Addition Test (PASAT 2 and 3);[24]and (7) changes in the familiarity and recognition functions in memory using the process dissociation procedure (PDP) test.[25]

Image acquisition

The MRI data were gathered from all 40 people. All images were acquired using a GE Signa HDxt 3.0T TX dualgradient dual-RF source MRI scanner and a head orthogonal eight-channel phased-array coil. High-resolution axial 3D T1-weighted images of the entire brain were obtained using a 3D fast spoiled gradient-recalled sequence. The scanning parameters were as follows: time of repetition 8.0 ms; time of echo 3.1 ms; inversion time 400 ms; thickness 1 mm; f lip angle 12°; matrix 240×240; number of excitation 1; and f ield of view 24 cm×18 cm. Moreover, 158 layers were scanned without interval.

Image analyses

FreeSurfer v6.0 (http://surfer.nmr.mgh.harvard.edu/)was used for image analysis. FreeSurfer is a powerful suite of tools that performs highly reliable and automated analysis of the human brain.[26]The analysis included volumetric segmentation of the brain structure and the segmentation of the hippocampus. FreeSurfer can accurately identify and segment cerebral cortex and subcortical structures in MRI imaging data and measure the changes of subpixel accuracy of cerebral cortex thickness with high sensitivity. FreeSurfer was used to segment the hippocampus and to obtain the volumes of different brain regions. The results of all scans and all automatic segmentation were visually examined to ensure proper skull dissection and correct segmentation.

Ethics statement

The Research Ethics Board of the Medical Center and Psychology Department of the Xi’an Jiaotong University approved this study.

综上所述，全国职业院校技能大赛学前教育专业教育技能大赛不仅是学前教育专业学生教育技能的比拼，更是《幼儿园指导纲要》与《3-6岁儿童学习与发展指南》的准教学实践解读，对于高职院校学前教育专业的人才培养方案以及课程教学改革都起到了引领和示范作用，相信在学前教育大发展的今天，学前教育专业教育技能大赛一定会为幼儿教师的培育和全面发展奠定坚实的教育实践基础。

RESULTS

Demographic information

There were no signif icant differences between the sepsis group and the healthy control group in general demographic data that included sex ratio, age, education level,hypertension, CHD, DM, tobacco use, and nonsteroidal antiinf lammatory drug (NSAID) use (Table 1).

Neuropsychological assessment

Figure 1 shows the results of clinical neuropsychological tests of different cognitive region functions. The results were tested by a normal distribution (Kolmogorov-Smirnov test).Data of the two groups were fitted to a normal distribution so that they could be compared using thet-test between two pairs of samples. According to the Wechsler Adult Intelligence Scale-III, no difference in the overall intelligence quotient (IQ) was found between the two groups (P＞0.05),a condition that could be used as a background to explain other areas of performance. In addition, we didn’t find any significant differences in visual assessment, executive function, or attention tests between the two groups (P＞0.05).Although the sepsis group showed a cognitive decline clinically, no significant difference was found compared with the healthy control group (P＞0.05). The levels of immediate free recall, immediate cued recall, and delayed cued recall in the CVLT-II were signif icantly lower in sepsis patients (P＜0.05). No significant difference in PASAT 2 or 3 was found between the two groups (P＞0.05). The explicit memory (recollection process) of the PDP test was lower in the sepsis group compared with the control group, but no signif icant difference was found in the implicit memory familiarity.

Structural neuroimaging

We used FreeSurfer v6.0 to automatically analyze and measure the volumes of different brain regions and hippocampal formation in the sepsis and control groups.The results indicated that the volumes of the left and right hippocampi in sepsis patients were significantly lower than those in the control group (P＜0.05). Compared with the control group, the volume of the presubiculum in the hippocampus of sepsis patients decreased, and the difference was statistically significant (P＜0.05). The volumes of CA1, CA3, CA4, molecular layer, granular dentate gyrus cell layer, and the hippo-amyg transitional area between the hippocampus and the amygdala were smaller in the sepsis group than in the healthy control group, but the differences were not statistically significant (P＞0.05). There was no significant difference in subcortical volume between the two groups, including the thalamus, caudate, putamen, pallidum, amygdala,and ventral diencephalon (P＞0.05), but we found that the subcortical volume in the sepsis group was smaller than that in the control group (Table 2).

Correlations between hippocampal volumes and neuropsychological tests

The APACHE II score of the sepsis group was negatively correlated with the volumes of left and right hippocampi (Figure 2A,r= -0.690,P＜0.05; Figure 2B,r= -0.694,P＜0.05), and the volumes of left and right hippocampi were positively correlated with immediate free recall (Figure 2C,r=0.496,P＜0.05; Figure 2D,r=0.526,P＜0.05), immediate cued recall (Figure 2E,r=0.484,P＜0.05;Figure 2F,r=0.497,P＜0.05), and delayed cued recall (Figure 2G,r=0.571,P＜0.05; Figure 2H,r=0.448,P＜0.05) of the CVLT-II (Figure 2). In the sepsis group, the hippocampal volume was significantly positively correlated with recollection but wasn’t correlated with familiarity (P＜0.05).No correlation was found between the hippocampal volume and CVLT-II in the healthy control group (P＞0.05), and between other cognitive tests and hippocampal volume in the two groups (P＞0.05).

DISCUSSION

Severe infection often causes neurological impairment. In a prospective cohort study, Yaffe et al[27]first reported a threefold increase in the risk of moderate-to-severe cognitive impairment after recovery from severe sepsis. A considerable number of patients develop abnormal neuropsychiatric function during hospitalization. After excluding direct infection of the central nervous system, head trauma, fat embolism, and adverse drug reactions that affect the state of consciousness, the diffuse brain dysfunction caused by the systemic inflammatory response can be diagnosed as SAE.[4,5]Many sepsis patients don’t receive comprehensive neuropsychological tests after discharge because they do not have obvious mental disorders during their hospital stay. Clinicians do not obtain a clear understanding of the nervous system function insepsis patients; thus, many patients do not receive the corresponding rehabilitation treatment. In the present study,the volume of the hippocampus in sepsis patients without neurological and psychiatric abnormalities was found to be reduced compared with that in the control group.

Table 1. Demographic and clinical characteristics of patients with sepsis and healthy controls

Table 2. The volume of different brain regions (mm3)

Azabou et al[28]found that an abnormal EEG and mental disorders could occur in the early stage of sepsis.The investigators further analyzed the data of sepsis patients in the recovery stage or discharged from the hospital about one month after treatment. Thus, in our study, we performed neuropsychological tests in participants within one month after enrollment.

Several neuropsychological tests on cognitive functions were performed, including visual, attention, memory,executive, and working memory. Although no differences in IQ, visual assessment, executive function, or attention test were found between sepsis patients and controls, sepsis patients exhibited a lower relative performance in immediate free recall, immediate cued recall, and delayed cued recall.Sepsis increases the risk of dementia[29-31]that severely affects the integrity of cognition and function[32]and reduces the quality of life.[30]In this study, no significant decline in the executive and attention functions was found in the early stage after sepsis patients were discharged from the hospital.This finding indicated that the impairment of executive and attention functions might not be an acute process, and rehabilitation training might delay the decline in executive and attention functions, thereby improving the life quality of sepsis patients.

Figure 1. Results of clinical neuropsychological tests of different cognitive region functions between the two groups. WAIS-III: Wechsler Adult Intelligence Scale-III; HVOT: Hooper Visual Organization Test; CVLT: California Verbal Learning Test; WCST: Wisconsin Card Sorting Test;PASAT: Paced Auditory Serial Addition Test; PDP: process dissociation procedure.

Figure 2. Correlations between APACHE II scores and hippocampal volume (A and B) and between estimates of neuropsychological test results and hippocampal volumes (C-H) in the sepsis group (n=20).

The memory function of sepsis patients, immediate free recall, immediate cued recall, or delayed cued recall declined significantly. The CA1 area and presubiculum of the hippocampus were related to the learning and memory functions.[31]The integrity of hippocampal neuronal structure and function is the premise of maintaining learning and memory; the decline in memory function in sepsis patients may be related to the decrease in the hippocampal volume.

The hippocampal volume was signif icantly correlated with recollection, but not with familiarity. The memory function of the hippocampus was controversial.[34,35]Some differences existed between familiarity and recollection as two different components of memory. The f indings of this study supported the idea that the hippocampus had a function in recollection. The volume of the presubiculum in the hippocampus decreased in sepsis patients,indicating that recollection was related to this area.

Because of the effects of various inflammatory factors, sepsis patients often develop hypotension leading to insufficient cerebral perfusion and changes in brain microcirculation.[4,5]The neuronal damage after cerebral ischemia is region-selective, and the vulnerability of neurons in sensitive areas is also different during cerebral ischemia and hypoxia.[14]In this study, the volume of the presubiculum decreased in the sepsis patients, and this was consistent with the results of previous studies. The main subjects of previous studies were patients with whole brain chronic hypoxia or acute hypoxia. The findings of our study indicated that the whole brain hypoxic condition may exist in sepsis instead of a localized injury caused by local exposure to inf lammatory factors alone.

These three results indicate that the factors that cause intracranial injury in sepsis patients may be more complicated than simple ischemia and hypoxia.

The volume of the presubiculum, which was not a vulnerable area of patients, also decreased in the areas considered to be vulnerable to ischemia. These results indicated that the factors, which caused intracranial injury in sepsis patients, might be more complicated. Of course, we cannot rule out inaccuracies due to the small sample size, the clarity of imaging, and the FreesSufer software division of the hippocampal formation.

The present study mainly focused on sepsis patients who didn’t have an obvious nervous system dysfunction during hospitalization but had abnormal EEGs. Because SAE is often diagnosed by clinical manifestations, some sepsis patients with abnormal EEGs are not timely recognized with SAE. Although most patients have early memory impairment, they are often discharged without evaluated with neuropsychological measures, and they are not considered in need of rehabilitation training. The impairment gradually affects the executive and attention functions, which can lead to challenges in life after discharge. Therefore,more advanced tests are needed to identify neurological abnormalities in sepsis patients during hospitalization, and early rehabilitation of sepsis patients who are not diagnosed with SAE should not be neglected. This approach may contribute to an improvement in the life quality of patients.We also found that executive and attention dysfunctions did not exist in sepsis patients at an early stage, and thus, by taking some steps early, we may be able to prevent or slow the decline in executive and attention functions.

There were some limitations in our study. The control group in our study was composed of healthy people.Although they were matched with the sepsis group for basic features such as sex, age, educational level, hypertension,CHD, diabetes, smoking, and NSAID use, sepsis patients may also have some unknown high risk of infection.Meanwhile, the study was a single-center, limited-sample study, which might affect the reliability of the results.

CONCLUSIONS

In general, sepsis can lead to a decrease in the size of the hippocampus, accompanied by early memory deficits.More advanced tests are needed to identify neurological abnormalities in sepsis patients during hospitalization.Even if they do not develop neurological dysfunction during hospitalization, sepsis patients should be assessed neurologically and, if necessary, treated with appropriate rehabilitation after hospital discharge. This strategy may improve long-term nervous system prognosis and quality of life, and reduce the incidence of non-Alzheimer’s dementia.

Funding:This work was supported by the Shanxi Province Key Scientific and Technological Project (2016YFJH2-05), and the Youth Project of the Second Aff iliated Hospital of Xi’an Jiaotong University (YJ[QN]201523).

Ethical approval:The Research Ethics Board of the Medical Center and Psychology Department of the Xi’an Jiaotong University approved this study.

Conflicts of interest:The authors have no competing interests relevant to the present study.

Contributors:All authors read and approved the f inal version of the manuscript.