甘蔗蜡提取物多廿烷醇的降血脂机制研究

廖芳，陈伟伟

摘要：采用RT-PCR、Western Blot、L-[35S]放射性标记物掺入等方法，观察甘蔗蜡提取物多廿烷醇对Hep G2细胞羟甲基戊二酸辅酶A（HMG-CoA）还原酶mRNA转录及蛋白质表达量的影响，及其对HMG-CoA还原酶合成和降解的作用。结果表明，多廿烷醇降低HMG-CoA还原酶mRNA的转录和蛋白质的表达量，且作用效果随多廿烷醇浓度的升高而增强。此外，多廿烷醇抑制HMG-CoA还原酶的合成并加速其降解，多廿烷醇处理后HMG-CoA还原酶的半衰期明显缩短。

关键词：多廿烷醇；Hep G2细胞；羟甲基戊二酸辅酶A（HMG-CoA）还原酶

中图分类号：R285.5 文献标识码：A 文章编号：0439-8114（2013）05-1125-03

Study on the Hypolipidemic Mechanism of Policosanol Extracted from Sugarcane Wax

LIAO Fang，CHEN Wei-wei

（Department of Pharmacology， Gannan Medical University， Ganzhou 341000， Jiangxi， China）

Abstract： The effects of policosanol extracted from sugarcane wax on mRNA transcription and protein expression level， synthesis and degeneration of 3-hydroxy-3-methylglutarl-coenzyme （HMG-CoA） reductase in Hep G2 cell were determined by RT-PCR， Western Blot and L-[35S] radioactive isotope tracing technology. The results showed that the mRNA transcription and protein expression level of HMG-CoA reductase decreased with the increase of policosanol concentration（10，20，30 μg/L）. Policosanol also suppressed the synthesis of HMG-CoA reductases while sped up its degeneration； the half life of HMG-CoA reductases treated by policosanol was shortened obviously.

Key words： policosanol； Hep G2 cell； 3-hydroxy-3-methylglutarl-coenzyme （HMG-CoA） reductase