电针风池穴对实验性偏头痛大鼠脑血流影响研究

康咪　屈箫箫　邵娣　尹晓航

摘要：目的 观察电针风池穴对实验性偏头痛大鼠行为症状学及脑血流的影响。方法 选取合格SD大鼠40只，按体重均衡原则分为4组（对照组、模型组、风池穴电针组、非穴电针组4组），每组10只，雌雄各半。对照组：予正常抓取，不作处理;模型组：参照硝酸甘油型偏头痛大鼠模型复制方法造模，建模后不予处理;风池穴电针组（造模后大鼠）与非穴电针组（造模后大鼠）均予以电针治疗：2 Hz和100 Hz交替，疏密波，刺激时间为10 min，后用TS激光探头测试大鼠头部脑血流量（PU值）。结果 针刺后，风池穴电针组与非穴电针组均能降低大鼠耳红持续时间、前肢挠头持續时间及大鼠各时间段挠头次数，具有统计学意义（P<0.05，P<0.01），其中风池穴组优于非穴组，具有统计学意义（P<0.01）;针刺后，风池穴组及非穴组偏头痛大鼠的脑血流量均升高，且风池穴组明显优于非穴组，具有统计学意义（P<0.01）。结论 针刺风池穴及非穴均能降低大鼠耳红持续时间、前肢挠头持续时间及大鼠各时间段挠头次数，且风池穴组优于非穴组;针刺后均能有效改善偏头痛大鼠的脑血流量，风池穴组优于非穴组。

关键词：风池穴;非穴;实验性偏头痛;脑血流量

中图分类号R245.3 文献标志码：A 文章编号：1007-2349（2019）05-0070-03

【Abstract】Objective： To observe the effect of electroacupuncture at Fengchi acupoint on behavioral symptomology and cerebral blood flow in experimental migraine rats. Methods： 40 SD rats were selected and divided into 4 groups according to the principle of weight balance （control group， model group， Fengchi acupuncture group， non-acupoint electroacupuncture group ）， 10 rats in each group， half male and half female. The control group were given normal capture and no treatment. The model group was established by reference model of nitroglycerin-type migraine rat model replication and no treatment after modeling. The Fengchi acupuncture group （rats after modeling） and non- electroacupuncture group （rats after modeling） were treated with electroacupuncture， alternating 2Hz and 100Hz with sparse wave， and stimulation time was 10 min， and then the brain blood flow （PU value） of the rat head was tested with TS laser probe. Results： After acupuncture， the rats in the Fengchi acupuncture group and the non-acupoint electroacupuncture group could reduce the duration of ear redness， the duration of forearm scratching and the number of head scratching in each period， which was statistically significant （P<0.05， P<0.01）， in which the Fengchi acupoint group was superior to the non-acupoint group， which was statistically significant （P<0.01）. After acupuncture， the cerebral blood flow of the Fengchi acupoint group and the non-acupoint group migraine rats increased. The Fengchi acupoint group was significantly better than the non-acupoint group， which was statistically significant （P<0.01）. Conclusion： Acupuncture at Fengchi and non-acupoints can reduce the duration of ear redness， the duration of forearm scratching and the number of head scratching in rats. The Fengchi acupoint group is better than non-acupoint group. The electroacupuncture at Fengchi Point can improve the cerebral blood flow in migraine rats after acupuncture and the Fengchi acupoint group was superior to the non-acupoint group.