Modulation of valosin-containing protein by Kyoto University Substances (KUS) as a novel therapeutic strategy for ischemic neuronal diseases

Masayuki Hata, Hanako Ohashi Ikeda,

1 Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan

2 Neuroprotective Treatment Project for Ocular Diseases, Institute for Advancement of Clinical and Translational Science, Kyoto University Hospital, Kyoto, Japan

Modulation of valosin-containing protein by Kyoto University Substances (KUS) as a novel therapeutic strategy for ischemic neuronal diseases

Masayuki Hata1,2, Hanako Ohashi Ikeda1,2,*

1 Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan

2 Neuroprotective Treatment Project for Ocular Diseases, Institute for Advancement of Clinical and Translational Science, Kyoto University Hospital, Kyoto, Japan

How to cite this article:Hata M, Ikeda HO (2017) Modulation of valosin-containing protein by Kyoto University Substances (KUS) as a novel therapeutic strategy for ischemic neuronal diseases. Neural Regen Res 12(8):1252-1255.

Retinal ischemia causes several vision-threatening diseases, including diabetic retinopathy, retinal artery occlusion, and retinal vein occlusion. Intracellular adenosine triphosphate (ATP) depletion and subsequent induced endoplasmic reticulum (ER) stress are proposed to be the underlying mechanisms of ischemic retinal cell death. Recently, we found that a naphthalene derivative can inhibit ATPase activity of valosin-containing protein, universally expressed within various types of cells, including retinal neural cells, with strong cytoprotective activity. Based on the chemical structure, we developed novel valosin-containing protein modulators, Kyoto University Substances (KUSs), that not only inhibit intracellular ATP depletion, but also ameliorate ER stress. Suppressing ER stress by KUSs is associated with neural cell survival in animal models of several neurodegenerative diseases, such as glaucoma and retinal degeneration. Given that a major pathology of ischemic retinal diseases, other than intracellular ATP depletion, is ER stress-induced cell death, KUSs may provide a novel strategy for cell protection in ischemic conditions. Hence, we investigated the ef fi cacy of KUS121 in a rat model of retinal ischemic injury. Intravitreal injections of KUS121, which is clinically preferable route of drug administration in retinal diseases, signif i cantly suppressed inner retinal thinning and retinal cell death, and maintained visual functions. Valosin-containing protein modulation by KUS is a promising novel therapeutic strategy for ischemic retinal diseases.

adenosine triphosphatase; C/EBP homologous protein; central retinal artery occlusion; endoplasmic reticulum stress; neuroprotective therapy; retinal ganglion cell

Introduction

Of all human tissues, the retina has the highest oxygen demands, and retinal ischemia contributes to the pathologies of several vision-threatening diseases, including diabetic retinopathy, glaucoma, retinal artery occlusion, and retinal vein occlusion (Osborne et al., 2004; Zheng et al., 2007). These retinal diseases account for a large proportion of blindness. Among these ischemic conditions, central retinal artery occlusion (CRAO) causes sudden severe vision loss, resulting in permanent blindness (Hayreh, 2011). The incidence of CRAO is estimated to be around 1.9/100,000 in the United States (Leavitt et al., 2011), but is increasing due to population aging and increased lifestyle-related diseases (Park et al., 2014). Patients with CRAO typically present with sudden deterioration of vision without pain, and 80% of af f ected patients have a fi nal visual acuity of counting fi ngers or worse (Hayreh and Zimmerman, 2005).e most common cause of CRAO is thromboembolus in the dural sheath of the optic nerve, the narrowest part of the CRA (Hayreh, 2011). CRAO can also occur due to occlusive thrombus immediately posterior to the lamina cribrosa (Mangat, 1995).

Given that the CRA perfuses the inner retinal layers, namely the retinal ganglion cell (RGC) layer and inner nuclear layer, visual loss in the CRAO occurs due to loss of blood supply to the inner retina (Hayreh et al., 2004). Def iciency of oxygen and glucose supply to retinal cells hinders adenosine triphosphate (ATP) production, which is necessary for nervous conduction in RGCs, resulting in immediate visual dysfunction. Retinal ischemia lasting more than 6 hours causes neural cell death in the RGC layer and inner nuclear layer, and subsequent nerve fi ber dropouts (Hayreh et al., 2004). Aer thickening of the inner retina in the acute phase of CRAO, inner retinal atrophy gradually progresses, resulting in permanent visual loss. However, in most cases of CRAO, spontaneous partial recanalization of the occluded CRA occurs within 48 to 72 hours. Spontaneous reperfusion is important for continued blood supply to the surviving neural cells, and reperfusion injury may contribute to addi-tional neural cell death (Szabo et al., 1991).

Current strategies for the management of acute CRAO focus on inducing reperfusion of the retina, ocular massage, anterior chamber paracentesis, and fibrinolysis. However, these treatments have limited or no efficacy for improving vision, given the short retinal tolerance time frame (potentially within 6 hours of symptom onset) for any therapy to be ef f ective (Schrag et al., 2015). Moreover, as spontaneous reperfusion occurs within a few days, therapeutic strategies for delaying neural cell death may be important components of CRAO treatment. There is no clinically effective neuroprotective treatment for CRAO, but some neuroprotective approaches have been trialed, especially for glaucoma (Sena and Lindsley, 2017).e latter is characterized by progressive RGC apoptosis and possibly associated with compromised blood fl ow in the optic nerve.

Figure 1 Kyoto University Substances (KUS) suppress adenosine triphosphate (ATP) depletion and endoplasmic reticulum (ER) stress in tunicamycin-treated cells.

Figure 2 Ef f ects of intravitreal injections of Kyoto University Substance 121 (KUS121) on a retinal ischemic rat model.

In addition to intracellular ATP depletion, the involvement of subsequent induced endoplasmic reticulum (ER) stresshas been proposed as the underlying mechanism of ischemic neuronal cell death, which is probably apoptosis (Tajiri et al., 2004). Neuronal cells have a highly-developed ER. Ischemia is an ER stressor that induces cell death in neural cells. C/EBP homologous protein (CHOP) is an ER stress-induced molecule that is reported to be a major mediator of apoptosis in neural ischemia. New drugs or compounds with ER stress-reducing activities may have neuroprotective functions in ischemic diseases, and investigation into their ability to prevent or delay neural cell death induced by ischemic injury might be useful.

Kyoto University Substances Protect Cells under ER Stress-Inducing Conditions

Valosin-containing protein (VCP) is an AAA (ATPase Associated with diverse cellular Activities)-type ATPase protein, ubiquitously expressed within various types of cells, including retinal neural cells (Higashiyama et al., 2002; Watts et al., 2004; Johnson et al., 2010). In several neurodegenerative diseases, such as Inclusion Body Myopathy with Paget’s disease of bone and Frontotemporal Dementia (IBMPFD) and familial amyotrophic lateral sclerosis, VCP has been proposed to be a major contributor. Pathogenic VCPs show elevated ATPase activities in these conditions, compared with wild-type VCPs, indicating that the constitutive elevation of ATPase activity may be pathogenic. On the other hand, in addition to ATPase activity, there are many proposed cellular functions of VCP, such as proteasome-mediated protein degradation, ER-associated degradation, cell cycle control, membrane fusion, protein traf fi cking, and autophagy (Meyer et al., 2012; Wolf and Stolz, 2012). Knockdown of VCP, as well as overexpression of dominant-negative forms of VCP showing cellular toxicity, indicate that some VCP functions are crucial for cell survival (Ikeda et al., 2014).

Recently, we found that a naphthalene derivative can inhibit ATPase activity of VCP and show cytoprotective activity, thereby enabling the development of novel VCP modulators, KUSs, or small chemical compounds derived from naphthalene, selected from approximately 200 newly synthesized compounds based on VCP inhibition of ATPase activity (Ikeda et al., 2014). KUSs do not signif i cantly impair reported cellular functions of VCP (e.g., ER-associated degradation or proteasome-mediated protein degradation (Ikeda et al., 2014)) but do suppress the decrease in cellular ATP levels. These “VCP modulators” have strong neuroprotective effects, both morphologically and functionally,in vivoon various types of retinal cells, including retinal photoreceptor cells and RGCs (Ikeda et al., 2014; Hasegawa et al., 2016; Nakano et al., 2016). We showed that KUSs not only inhibit intracellular ATP depletion, but ameliorate ER stress in Hela cells treated with tunicamycin (Ikeda et al., 2014) (Figure 1). Among several KUSs, KUS121 had a strong cellular protective ef f ect duringin vitroexperiments using culture cells, including neural cells (Ikeda et al., 2014; Nakano et al., 2016), and were easily synthesized. Therefore, we mainly used KUS121 in subsequent experiments. ER stress can induce apoptosis in a diverse range of cell types, contributing to various diseases. Suppressing ER stress by KUSs was associated with enhanced neural cell survival in several neurodegenerative ocular diseases (Ikeda et al., 2014; Hasegawa et al., 2016; Nakano et al., 2016).

VCP Modulation by KUSs as a Promising Novelerapeutic Strategy for Ischemic Neuronal Diseases

Given that the major pathology of ischemic neural diseases is ER stress-induced cell death (Li et al., 2014), KUSs may provide a novel strategy for cell protection in ischemic conditions. Hence, we investigated the neuroprotective ef f ect of a KUS on the retina aer ischemic injury, using a rat model of ischemic retinal injury (Hata et al., 2017).e intraocular pressure of-green fl uorescent protein (GFP) rats, which express GFP in RGCs (Magill et al., 2010), was increased to 120 mmHg for 60 minutes to induce retinal ischemia (Figure 2A). We evaluated the ef fi cacy of intravitreal (IVT) injections of KUS, given that IVT is the clinically preferred route of drug administration in retinal diseases. In the group treated with IVT injections, KUS121 (25 µg/eye) or phosphate buffer saline (PBS) was injected into the vitreous ofThy1-GFP rats, using a 33-gauge needle, two hours before ischemic retinal injury.

We examined the time-dependent changes in inner retinal thickness in the eyes of-GFP rats with ischemic retinal injury using spectral domain-optical coherent tomography (SD-OCT), to detect retinal damage or atrophy caused by ischemic retinal injury. The inner retina, composed of the retinal nerve fi ber layer, RGCs, inner plexiform layer, and inner nuclear layer, located under the retinal vessel supply, was primarily impaired by ischemic insult (Murata et al., 2008). Notably, compared to the control group, the KUS121-treated group showed signif i cant suppression of inner retinal thinning 7 and 14 days aer ischemic insult (day 7,P= 0.0008; day 14,P= 0.0001; Figure 2B) (Hata et al., 2017). To evaluate the neuroprotective ef f ects of KUS121 on the RGC layer, we used a scanning laser ophthalmoscope (SLO) to measure the number of RGCs remaining after ischemic insult. IVT injections of KUS121 showed a preservation of GFP-positive RGCs 14 days aer the insult (P= 0.0078 Figure 2C and D) (Hata et al., 2017). For functional analysis, we performed electroretinography (ERG) and assessedb-wave amplitudes to confirm whether the neuroprotective effects of KUS121 were mirrored by the preservation of visual function after ischemic retinal injury (Block and Schwarz, 1998). KUS121 preservedb-wave amplitude 14 days aer the insult (before the insult,P= 0.861; aer the insult,P= 0.0018; Figure 2E) (Hata et al., 2017). To examine the mechanisms underlying the pathological and KUS treatment processes of ischemic retinal injury, we examined ER stress and apoptosis markers aer ischemic insult (Hata et al., 2017). CHOP and cleaved caspase-3 proteins were induced after ischemic injury. The CHOP and cleaved caspase-3 proteins levels were significantly decreased in the ischemic retinal-injured rats treatedwith KUS121 (Hata et al., 2017). TdT-mediated dUTP Nickend Labeling (TUNEL) staining of retinal sections conf i rmed that there were fewer apoptotic cells in the RGC layer and inner nuclear layer of the KUS121-treated group than in the control group (Hata et al., 2017).erefore, KUS121 exerts not only morphological but also functional neuroprotective ef f ects on the inner retina during retinal ischemia, thereby demonstrating the potential of KUS121 in the treatment of ischemic retinal diseases. To test the safety and efficacy of KUS121 on CRAO patients, we are now performing an investigator-initiated clinical trial.

Statistical analyses were performed using SPSS Statistics version 17.0 (SPSS Inc, Chicago, IL, USA). Variables among cells or rats treated with or without KUSs were compared with the Dunnett’s test or Student’st-test.

Conclusion

We showed that the VCP modulator KUS121 exerts an anti-apoptotic ef f ect on ischemic retinal injury by suppressing ER stress, resulting in morphological and functional neuroprotection. Our fi ndings may provide a novel therapeutic strategy for the treatment of neural ischemic diseases. Furthermore, given that ER stress is also the major underlying pathology of many other incurable disorders, such as neurodegenerative diseases, KUSs may provide a novel strategy for cell protection in such conditions.

Author contributions:

Conf l icts of interest:Kyoto University has applied for patents related to this study (PCT/JP2015/055619 & PCT/JP2011/073160), with Masayuki Hata and Hanako Ohashi Ikeda listed as inventors. Masayuki Hata and Hanako Ohashi Ikeda are currently performing a joint research project in preparation for an investigator-initiated clinical trial with Kyoto Drug Discovery and Development Co., Ltd.

Plagiarism check:Checked twice by ienticate.

Peer review:Externally peer reviewed.

Open access statement:

Open peer review report:

Reviewer: Miguel A. Marin, University of California Los Angeles, USA.

Additional fi le: Open peer review report 1.

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*< class="emphasis_italic">Correspondence to: Hanako Ohashi Ikeda, M.D., Ph.D., hanakoi@kuhp.kyoto-u.ac.jp.

Hanako Ohashi Ikeda, M.D., Ph.D., hanakoi@kuhp.kyoto-u.ac.jp.

orcid: 0000-0001-9572-8659 (Hanako Ohashi Ikeda)

10.4103/1673-5374.213540

Accepted: 2017-07-20