长期饲喂高精料日粮导致的SARA效应对泌乳期山羊乳品质及肝脏中基因表达的影响
2016-05-14董海波倪迎冬
董海波 倪迎冬
摘 要:选择8头安装瘤胃瘘管的泌乳中期山羊随机分为2组分别饲喂高精料日粮(60%干物质,HC组)和低精料日粮(40%干物质,LC组),实验周期9周,期间采集瘤胃液,血液及肝脏组织。研究长期饲喂高精料日粮后肝脏中代谢底物的变化规律。实验结果显示:饲喂9周后,高精料日粮导致瘤胃液pH显著下降;乳脂,乳蛋白也显著下降;血浆中瘦素leptin(P=0.05),非酯化脂肪酸NEFA(P=0.07),葡萄糖glucose(P=0.014)均显著上升;血浆皮质醇cortisol(P<0.05)水平显著上升。肝脏中糖皮质激素受体GR蛋白与基因表达一致均显著显著下调(P<0.05);IL-1β,SAA,CRP及HP基因表达均显著上升(P<0.05)。高通量芯片分析显示与炎症反应,脂代谢,蛋白代谢以及糖代谢的关键基因均发生显著上调或下调,代谢调控网络发生变化。结论 急性期反应与炎症反应可能促进肝脏中能量重分配并最终导致乳品质下降。
关键词:乳品质 急性期反应 炎症反应 代谢 基因表达 肝脏 泌乳山羊
Long-term Effects of SARA on Milk Quality and Hepatic Gene Expression in Lactating Goats Fed a High-concentrate Diet
Dong Haibo Ni Yingdong
(Nanjing Agricultural University)
Abstract: Eight mid-lactating goats with rumen cannulas were randomly assigned to two groups. For 9 weeks, the treatment group was fed a high-concentrate diet (60% concentrate of dry matter, HC) and the control group was fed a low-concentrate diet (40% concentrate of dry matter, LC). Ruminal fluid, plasma, and liver tissues were sampled, microarray techniques and real-time polymerase chain reaction were used to evaluate metabolic parameters and gene expression in liver. Feeding a 60%-concentrate diet for 9 weeks resulted in a significant decrease in rumen pH. Changes in fat and protein content also occurred, which negatively affected milk quality. Plasma levels of leptin (P=0.058), non-esterified fatty acid (P=0.071), and glucose (P=0.014) increased markedly in HC group. Plasma cortisol concentration was significantly elevated in the treatment group (P<0.05). Expression of the glucocorticoid receptor protein gene was significantly down-regulated (P<0.05) in the liver. The expression of genes for interleukin 1b, serum amyloid A, C-reactive protein, and haptoglobin mRNA was significantly increased (P<0.05) in the HC group. GeneRelNet analysis showed that gene expression involved in inflammatory responses and the metabolism of lipids, protein, and carbohydrate were significantly altered by feeding a high-concentrate diet for 9 weeks.
Key Words: Milk quality; Acute phase response; Inflammatory response; Metabolism; Gene expression; Liver; lactating goats
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