甲基对硫磷残留酶联免疫检测试剂盒的研制

万宇平，孙震，冯静，扶胜，崔廷婷，齐向武

摘要：合成了甲基对硫磷的半抗原，并与载体蛋白偶联合成了人工抗原，然后通过免疫的方法制备了其单克隆抗体。在筛选甲基对硫磷单克隆抗体的基础上，建立酶联免疫检测方法，并研制出检测苹果、玉米、蔬菜中甲基对硫磷残留的试剂盒。该试剂盒的灵敏度为0.5 μg/L，对苹果、玉米、蔬菜样品的最低检测限分别可达到10、20、50 μg/kg；加标回收率为70.3%～102.4%，变异系数为7.4%～13.0%；对实际样品的检测结果与气相色谱法基本一致。该方法快速、灵敏、可靠，为水果、蔬菜、谷物中甲基对硫磷的残留检测提供了便捷、准确的分析手段。

关键词：甲基对硫磷；残留；酶联免疫试剂盒

中图分类号：S482.3；Q511 文献标识码：A 文章编号：0439-8114（2013）05-1154-04

Development of an Enzyme-linked Immunosorbent Assay Kit for Detecting of Parathion-methyl Residues

WAN Yu-ping，SUN Zhen，FENG Jing，FU Sheng，CUI Ting-ting，QI Xiang-wu

（Beijing Kwinbon Biotechnology Co. Ltd.，Beijing 102206，China）

Abstract：A hapten artificial antigen was prepared， and monoclonal antibodies against parathion-methyl were produced. On the basis of parathion-methyl monoclonal antibody， an enzyme-linked immunosorbent assay（ELISA） kit for parathion-methyl residues in apple， corn and vegetable was developed. The sensitivity of the ELISA kit was 0.5 μg/L. The detection limits for apple， corn， vegetable were 10， 20， 50 μg/kg， respectively. Sample recoveries were 70.3%～102.4%， and the coefficients of variation were 7.4%～13.0%. Results of the ELISA kit were in accordance with those by gas chromatography（GC）. This method is rapid， sensitive and reliable， which provides a convenient and accurate way for detection of parathion-methyl residues in fruit， cereal and vegetable.

Key words： parathion-methyl； residue； enzyme-linked immunosorbent assay （ELISA） kit