罗汉果组培苗二次脱毒技术研究

吴群英，李伯林，李景云

摘要：对罗汉果（Siraitia grosvenorii）组培苗采用热处理和剥取茎尖技术进行二次脱毒处理，脱毒效果采用间接酶联免疫法和电镜观察法进行检测。结果表明，适合材料热处理的培养基为MS+0.3 mg/L 6-BA+NAA 0.1 mg/L+0.1 mg/L IBA，热处理材料的顶芽呈锥形，利于剥取茎尖；茎尖分化最佳培养基为MS+0.3 mg/L 6-BA +0.05 mg/L NAA+0.1 mg/L GA3；38.5 ℃热处理10 d剥取长1.0～1.5 mm的茎尖进行二次脱毒培养，存活率为80.00%，脱毒率为100.00%。

关键词：罗汉果（Siraitia grosvenorii）花叶病毒；茎尖；热处理；脱毒；酶联免疫法

中图分类号：S668.9 文献标识码：A 文章编号：0439-8114（2013）05-1185-03

Study on Duplicate Virus-free Techniques of Siraitia grosvenorii

Tissue Culture Seedling

WU Qun-ying1，LI Bo-lin2，LI Jing-yun3

（1. Biotechnology College， Guilin Medical University， Guilin 541004， Guangxi， China；

2. College of Life Science， Guangxi Normal University， Guilin 541004， Guangxi， China；

3. College of Basic Medicine， Guangxi University of Chinese Medicine， Nanning 530001，China）

Abstract： The tissue culture seedlings of Siraitia grosvenorii were treated by heat treatment and tip-stripping to obtain virus-free seedling. ELISA（enzyme-linked-immunosorbent serologic assay） method and electron microscope method were applied for virus detection. The results showed that the suitable medium for heat treatment was MS+0.3 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L IBA. Terminal bud after heat treatment was conical and easy for stripping stem tip. The optimum medium for inducement of tips was MS+0.3 mg/L 6-BA+0.05 mg/L NAA+0.1 mg/L GA3. Heat treatment for 10 d at 38.5 ℃ twice， stripping shoot-tip for 1.0～1.5 mm long， the survival rate was 80.00%； and the detoxication rate was 100.00%.

Key words： Siraitia grosvenorii virus （WMV-2-Luo）； shoot-tip； heat treatment； virus-free； ELISA