热应激对兔下丘脑-垂体-睾丸轴NMB及其受体基因表达和睾酮分泌的影响

马志禹 张莹 陈风雷 杨盛 乔文娜 胡佳慧 张金龙 雷治海

摘要：研究热应激对公兔下丘脑-垂体-睾丸（HPT）轴神经介素B（NMB）及其受体（NMBR）基因表达水平及血清皮质醇和睾酮分泌的影响，探讨热应激对神经肽和相关激素分泌的影响。建立热应激模型，采用荧光定量PCR（RT-PCR）检测兔NMB和NMBR mRNA在HPT轴的表达情况，采用放射免疫分析法（RIA）检测兔血清中皮质醇和睾酮浓度的变化规律。结果表明，兔NMB和NMBR基因在HPT轴中表达;NMB和NMBR mRNA在HPT轴的表达模式相似，且热应激后在一定程度上可以促进NMB和NMBR基因的表达，并按时序在下丘脑（热应激0.5 h）、垂体（热应激1.0 h）和睾丸（热应激2.0 h）促进作用最为明显;热应激可以促进皮质醇的分泌，并抑制睾酮的分泌，但这种抑制作用随着热应激时间的增加（热应激2.0 h）逐渐恢复正常。表明热应激可能通过NMB/NMBR系统调节HPT轴的功能，进而影响动物繁殖。

关键词：热应激;神经介素B;NMBR;下丘脑-垂体-睾丸轴;睾酮

中图分类号：Q78         文献标识码：A

文章编号：0439-8114（2019）12-0141-06

DOI：10.14088/j.cnki.issn0439-8114.2019.12.033           開放科学（资源服务）标识码（OSID）：

Abstract： The aim of this study was to detect the effect of heat stress on the expression levels of Neuromedin B （NMB） and its receptor （NMBR） genes in the hypothalamus-pituitary-testis（HPT） axis of male rabbit， and the secretion of serum cortisol （Cor.） and testosterone （T）. Establishment The heat stress model was established， the expression levels of NMB and NMBR genes in rabbit HPT axis were detected by real-time PCR（RT-PCR）， and the concentration of Cor. and T in rabbit serum were tested using radioimmunoassay （RIA）. Results showed that NMB and NMBR genes were expressed in rabbit HPT axis. The expression pattern of NMB and NMBR mRNA in HPT axis were similar， and heat stress could promote the NMB and NMBR gene expression， which the role was mostly obvious according to the time sequence in the hypothalamus （heat stress 0.5 h）， pituitary （heat stress 1.0 h） and testis （heat stress 2.0 h）; RIA results indicated that heat stress could improve the concentration of Cor. and inhibit T secretion which the inhibition was gradually weakened over time of heat stress. The results indicated that heat stress may regulate the HPT axis through the NMB/NMBR system to affect reproductive function， and then affected the reproduction of animals.

Key words： heat stress; neuromedin B; neuromedin B receptor; hypothalamus-pituitary-testis axis; testosterone

收稿日期：2018-11-05

作者简介：马志禹（1988-），男，河南许昌人，讲师，博士，从事神经肽及其受体、神经内分泌与生殖等方面的研究，