鼠尾草酸对大鼠大脑中动脉阻断引起的局灶性脑缺血的保护作用

金成 张兰春 于浩飞

【摘 要】 目的：研究鼠尾草酸預处理对大鼠大脑中动脉阻断引起的局灶性脑缺血的保护作用。方法：将健康雄性SD大鼠随机分为7个组：假手术组、模型组、尼莫地平组、灯盏花素组、鼠尾草酸180mg/kg鼠尾草酸90mg/kg组、鼠尾草酸45mg/kg组。各组大鼠连续灌胃给药7d，末次给药后，采用改良线栓法制备大鼠大脑中动脉栓塞（Middle Cerebral Artery Occlusion， MCAO）模型，观察鼠尾草酸预处理对大鼠大脑中动脉阻断引起的局灶性脑缺血的影响。结果：鼠尾草酸180mg/kg组能明显缩小脑梗死面积百分比，抑制血小板聚集，显著性降低纤维蛋白原（FIB）含量;鼠尾草酸90mg/kg组能明显改善大鼠神经损伤症状，缩小脑梗死面积百分比，延长活化部分凝血活酶时间（Activated Partial Thromboplastin Time，ATPP），降低纤维蛋白原（Fibrinogen，FIB）;鼠尾草酸45mg/kg组能延长ATPP，明显降低纤维蛋白原（FIB）含量。此外，血常规结果显示，不同剂量鼠尾草酸预处理对MCAO大鼠的的各项生理指标起到一定的调节作用，差异具有统计学意义。结论：鼠尾草酸预处理对大鼠大脑中动脉阻断引起的局灶性脑缺血的具有良好的保护作用。

【关键词】 鼠尾草酸;MCAO;血小板聚集率;血常规检测

【中图分类号】 R285 【文献标志码】 A【文章编号】1007-8517（2019）1-0010-07

Abstract：Objective Our aim is to study the role of carnosic acid preconditioning on focal cerebral ischemia caused by middle cerebral artery occlusion in rats.Methods  Healthy male Sprague-Dawley rats were randomly divided into 7 groups： sham operation group， model group， nimodipine group， breviscapine group， carnosic acid 180  mg/kg group， carnosic acid 90 mg/kg group， and carnosic acid 45 mg/kg group.Rats in each group were administered with intragastric administration for 7 days continuously. After the last administration， the Middle Cerebral Artery Occlusion（MCAO）model of rats were prepared by modified suture method to observe the influence of focal cerebral ischemia caused by the treatment of rat middle cerebral artery occlusion. Results  The percentage of cerebral infarction area were significantly reduced， the aggregation of platelets were inhibited， and fibrinogen （FIB） content dramatically was decreased in carnosic acid 180mg/kg group. Then the symptoms of nerve damage in rats were remarkably improved， the percentage of cerebral infarction were reduced， the activated partial thromboplastin time （ATPP）， and decrease fibrinogen （FIB） content were lengthened in carnosic acid 90mg/kg group， compared with model group. Ultimately， the ATPP and fibrinogen （FIB） content of carnosic acid 45mg/kg group were longer and lower than model group. In addition， blood routine test results showed that different doses of carnosic acid pretreatment had certain regulation effects on various physiological indexes of middle cerebral artery occlusion （MCAO） rats， and the difference was statistically significant among the groups. Conclusions  Therefor， the pretreatment of carnosic acid had a good protective effect on focal cerebral ischemia caused by middle cerebral artery occlusion in rats.

Keywords：Carnosic Acid; MCAO; Platelet Aggregation; Blood Routine Test