不同矿物成因禹余粮矿物成分分析及止血作用研究

吴超颖+刘圣金+房方+王宇华+杨文国+马瑜璐+吴德康

[摘要]小鼠采用华法林钠造模，用断尾法和玻片法分别测定出血时间和止血时间，大鼠连续15 d给药测定血浆复钙时间、血浆黏度、血小板黏附率、血小板聚集率等血液指标。 与空白组比较，模型组能延长（P<005）出血时间，与模型组相比阳性维生素K、淋滤浸染型水煎液和沉积型水煎液能显著（P<001）缩短出血时间，阳性维生素K能显著（P<001）缩短凝血时间，淋滤浸染型水煎液、沉积型水煎液和沉积型粉末可（P<005）缩短凝血时间。与空白组比较，淋滤浸染型水煎液低剂量、中剂量和粉末中剂量，以及沉积型水煎液高剂量和药渣低剂量都可降低血浆黏度（P<005），淋滤浸染型粉末高剂量和沉积型水煎液低剂量能显著降低（P<005）血浆黏度。与空白组比较，禹余粮淋滤浸染型水煎液高剂量组能显著降低（P<005）血小板黏附率，沉积型水煎液高能显著增加（P<005）血小板黏附率，淋滤浸染型水煎液高剂量、药渣高剂量和淋滤浸染型粉末低剂量、药渣低剂量能降低（P<005）血小板聚集率，沉积型水煎液高剂量、粉末高剂量能增加（P<005）血小板聚集率。矿物成分分析利用偏光显微镜及X射线衍射（Xray diffraction，XRD）法。 2种矿物成因禹余粮矿物组成均含针鐵矿、石英、高岭石，沉积型尚含伊利石、钠长石。研究结果表明沉积型成因的禹余粮止血作用较好，可能与针铁矿、伊利石含量较高有关。

[关键词]矿物药； 禹余粮； 矿物成因； 止血； 凝血； 矿物成分

Mineral composition analysis and hemostatic

effect of limonitum with different mineral genesis

WU Chaoying， LIU Shengjin， FANG Fang， WANG Yuhua， YANG Wenguo， MA Yulu， WU Dekang*

（Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， National and Local Collaborative

Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine， State Administration of

traditional Chinese medicine Key Laboratory of Chinese Medicine Resource Recycling Utilization， Jiangsu Key Laboratory for

Functional Substance of Chinese Medicine， Nanjing University of Chinese Medicine， Nanjing 210023， China）

[Abstract]Models were established in mice with warfarin sodium method， and their bleeding time and hemostasis time were measured by tail cutting method and slide method respectively Rats were administered for 15 consecutive days to measure their recalcification time， plasma viscosity， platelet adhesion rate， platelet aggregation rate and other blood indexes As compared with the blank group， the bleeding time was prolonged in model groupn（P<005） As compared with the model group， the results showed that the positive vitamin K， the leaching type water decoction and the sediment type decoction could significantly shorten the bleeding time （P<001）； positive vitamin K significantly （P<001） shortened clotting time， and the leaching type water decoction， the sediment type water decoction and the sediment type powder could also shorten the clotting time （P<005） As compared with blank group， low dose， medium dose of leaching type water decoction， medium dose of powder， high dose of sediment type decoction and low dose of drug residues could reduce plasma viscosity （P<005）， and high dose of leaching powder and low dose of water decoction could significantly reduce （P<001） plasma viscosity As compared with blank group， Limonitum leaching type decoction high dose group could significantly reduce the platelet adhesion rate （P<005）， while sediment type water decoction could significantly increase the platelet adhesion rate （P<005）； the high dose of leaching type water decoction， high dose of drug residues， low dose of leaching type powder and low dose of drug residues could decrease the platelet aggregation rate （P<005）， while high dose of leaching type water decoction and high dose of the powder could increase the platelet aggregation rate （P<005） Analysis of mineral compositions was conducted by polarized light microscopy and Xray diffraction （XRD） The results of the both methods showed that Limonitum mineral compositions contained goethite， quartz， and kaolinite， and sedimentary type also contained illite and albite Sediment type of Limonitum showed better hemostatic effect， which may be related to the high content of goethite and illite.endprint