芪葵颗粒对糖尿病肾病大鼠肾脏中TGF—β1及MCP—1表达的影响

杨晨+朱萱萱++余江毅++张迪

[摘要] 目的 觀察芪葵颗粒对糖尿病肾病大鼠肾脏中转化生长因子β1（TGF-β1）及单核细胞趋化蛋白1（MCP-1）的影响，初步探讨其作用机制。 方法 本研究采用高脂高糖饲料喂养+单侧肾结扎+腹腔注射2%链脲佐菌素（STZ）造成糖尿病肾病大鼠模型，成模后将其随机分为模型组，厄贝沙坦组，芪葵颗粒高、中、低剂量组，另设假手术组，每组10只，连续灌胃给药8周。血糖仪测定大鼠血糖值，酶联免疫吸附测定（ELISA）法检测血清中白细胞介素6（IL-6）的含量，免疫组化法检测肾组织中MCP-1、TGF-β1的表达。 结果 与模型组比较，芪葵颗粒高、中剂量组大鼠血糖显著降低，差异有统计学意义（P < 0.05）；与实验前比较，芪葵颗粒高、中、低剂量组大鼠血糖均显著降低（P < 0.01或P < 0.05）；与模型组比较，芪葵颗粒高、中、低剂量组大鼠血清中IL-6含量明显降低（P < 0.01），肾组织中MCP-1、TGF-β1的表达明显减少（P < 0.01）。 结论 芪葵颗粒对糖尿病肾病大鼠肾组织具有较好的保护作用，其作用机制可能与其降低血糖、减少IL-6和抑制MCP-1、TGF-β1的表达有关。

[关键词] 芪葵颗粒；糖尿病肾病；白细胞介素6；单核细胞趋化蛋白1；转化生长因子β1

[中图分类号] R587；R692.3 [文献标识码] A [文章编号] 1673-7210（2016）12（b）-0008-04

Effect of Qikui Granules on the expression of TGF-β1 and MCP-1 in kidney of rats with diabetic nephropathy

YANG Chen ZHU Xuanxuan YU Jiangyi ZHANG Di

Pharmacology Laboratory， Affiliated Hospital of Nanjing University of Chinese Medicine， Jiangsu Province， Nanjing 210029， China

[Abstract] Objective To observe the effects of Qikui Granules on the expression of transforming growth factor β1 （TGF-β1） and monocyte chemotactic protein 1 （MCP-1） in kidney of rats with diabetic nephropathy， and to evaluate the mechanism. Methods This experiment adopted the high-sugar-high-fat diet + unilateral renal ligation + intraperitoneal injection of 2% STZ to establish rat models with diabetic nephropathy， then the rats were randomly divided into model group， Irbesartan group， Qikui Granules of high， medium， low dose group， and sham group， with 10 rats in each group. Continuous intragastric administration of 8 weeks was taken. The values of blood glucose in rats were measured by blood glucose meter. The contents of serum interleukin 6 （IL-6） were determined by enzyme linked immunosorbent assay （ELISA）. The expression of MCP-1 and TGF-β1 in kidney was evaluated by immunohistochemistry method. Results Compared with model group， the levels of blood glucose in Qikui Granules of high， medium dose groups were decreased （P < 0.05）. The levels of blood glucose in Qikui Granules of high， medium， low dose groups were significantly lower than those before experiment （P < 0.01 or P < 0.05）. Compared with model group， the contents of IL-6 in Qikui Granules of high， medium， low dose groups were significantly decreased （P < 0.01）， the expression of MCP-1 and TGF-β1 in kidney of Qikui Granules of high， medium， low dose groups was significantly reduced （P < 0.01）. Conclusion Qikui Granules have better protective effects for the renal tissue of rats with diabetic nephropathy. Its mechanism may be related to the decrease of blood glucose and IL-6， the inhibition of the expression of MCP-1 and TGF-β1.