四物汤及活血、养血药对慢性阻塞性肺疾病气道重塑及黏膜免疫的影响

陈静+程羽+夏永辉+叶志鹏+袁嘉丽

[摘要] 目的 探討四物汤及其活血、养血药对慢性阻塞性肺疾病（COPD）气道重塑及黏膜免疫的作用机制。 方法 8周龄SPF级Wistar雄性大鼠50只，按体重分层随机分为正常对照、模型组、四物汤组、活血药对组、养血药对组，每组各10只。采用烟熏加脂多糖滴注复制COPD大鼠模型，四物汤组、活血药对组、养血药对组于造模成功后给予对应中药2 mL/（只·d）灌胃，连续4周，模型组给予等量生理盐水灌胃。正常对照组自由饮水。末次给药24 h后处死各组大鼠，取左肺进行病理检查，取右肺组织匀浆以ELISA法检测分泌型免疫球蛋白A（SIgA）、分泌片（SC）、转化生长因子β1（TGF-β1）、血管内皮生长因子（VEGF）的表达量。 结果 模型组大鼠肺组织病理显示，炎性细胞浸润明显，气道结构紊乱，管腔狭窄，气道壁纤维组织增生明显，管壁增厚，肺泡腔扩张，肺泡腔融合；药物干预后，四物汤、活血、养血药对各组肺组织病理均有不同程度改善。模型组SIgA、SC、TGF-β1、VEGF的表达量较正常对照组显著升高（P < 0.01）；药物干预各组较模型组SIgA、SC、TGF-β1、VEGF均显著降低（P < 0.01）。 结论 SIgA、SC、TGF-β1、VEGF参与COPD的发生发展过程；四物汤、活血、养血药对可通过调节SIgA、SC、TGF-β1、VEGF的表达干预COPD黏膜免疫平衡及气道重塑进程。

[关键词] 四物汤；活血药对；养血药对；气道重塑；黏膜免疫

[中图分类号] R563 [文献标识码] A [文章编号] 1673-7210（2017）03（a）-0008-05

Effects of Siwu Tang and Huoxue， Yangxue drugs on airway remodeling and mucosal immunity in rats with chronic obstructive pulmonary disease

CHEN Jing CHENG Yu XIA Yonghui YE Zhipeng YUAN Jiali

School of Basic Medical Science， Yunnan University of Traditional Chinese Medicine， Yunnan Province， Kunming 650500， China

[Abstract] Objective To explore the mechanism of Siwu Tang and Huoxue， Yangxue drug pairs in airway remodeling and mucosal immunity of rats with chronic obstructive pulmonary disease （COPD）. Methods 50 Wistar male healthy rats of SPF grade aged 8 weeks old were randomly divided into the normal control group， COPD model group， Siwu Tang group， Huoxue drug pair group， Yangxue drug pair group， according to the body weight of rats. The rats model of COPD was induced by smoke and lipopolysaccharide （LPS） infusion. The normal control group was given free drank water. After the model successfully established， the corresponding Chinese medicine 2 mL/（rat·d） was given by gavage in the drug groups for 4 weeks. The model group was given normal saline by the same way. 24 h after last administration， the rats of each group were sacrificed and the specimens were collected. Subsequently the pathological changes of the lung tissue were observed， and the levels of secretory immunoglobinA （SIgA）， secretory component （SC）， transforming growth factor-β1 （TGF-β1）， vascular endothelial growth factor （VEGF） in lung tissue were detected by ELISA. Results The pathology of rats lung tissue showed that airway inflammatory cells infiltrated in airway tissue， vessel stenosis， bronchial smooth muscle increased thickness， alveolar dilation and fusion in COPD model group； the pathology of the lung tissue changed better in Siwu Tang group， Huoxue drug pair group and Yangxue drug pair group. Compared with the normal control group， the levels of SIgA， SC， TGF-β1， VEGF increased in the COPD model group （P < 0.01）； after using corresponding traditional Chinese medicine， compared with the COPD model group， the levels of SIgA， SC， TGF-β1， VEGF decreased significantly （P < 0.01）. Conclusion SIgA， SC， TGF-β1， VEGF involves in the development of COPD. Siwu Tang， Huoxue and Yangxue drug pairs improve the mucosal immune balance and airway remodeling process in COPD by regulating the level of SIgA， SC， TGF-β1， VEGF.