Ginsenoside metabolite compound K alleviate collegen-induced arthritis through impairing dendritic cells function

Jing-yu CHEN,Hua-xun WU,Qing-tong WANG,Yan CHANG,Kang-kang LIU,Wei WEI

(Institute of Clinical Pharmacology,Anhui Medical University,Key Laboratory of Anti-inflammatory and Immune Medicine,Ministry of Education,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine,Hefei 230032,China)

T2-3

Ginsenoside metabolite compound K alleviate collegen-induced arthritis through impairing dendritic cells function

Jing-yu CHEN,Hua-xun WU,Qing-tong WANG,Yan CHANG,Kang-kang LIU,Wei WEI

(Institute of Clinical Pharmacology,Anhui Medical University,Key Laboratory of Anti-inflammatory and Immune Medicine,Ministry of Education,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine,Hefei 230032,China)

OBJECTIVEGinsenoside metabolite compound K(CK)is a degradation product of ginsenoside in the intestine by bacteria.The anti-inflammatory and immunomodulatory activities of CK have been reported.This study investigated whether CK exerted its immunoregulatory effect through modulation of dendritic cells(DCs)function.METHODSIn vivo,severity of collegen-induced arthritis(CIA),T cells and DCs subsets,phenotype of DC were assayed by flow cytometry,CCL19 and CCL21 level in lymph nodes assayed by ELISA.In vitro,bone marrow-derived DCs from normal mice were matured with lipopolysaccharide and treated with CK for 48 h.In vivo,bone marrow-derived DCs were generated from CIA mice before and 2 weeks into CK treatment.DCs were analyzed for migration,phenotype and T-cell stimulatory capacity.RESULTSCK alleviated the severity of CIA,decreased pDCs and mo-DCs,increased naïve T cells in CIA mice lymph nodes,and suppressed CCL21 expression in lymph nodes.CK suppressed DCs migration induced by CCL21 and T cells-stimulatory capability of DC,down-regulated LPS-induced expression of CD80,CD86,MHCII and CCR7 on DCs.CONCLUSIONThis study elucidated the novel immunomodulatory property of CK via impairing function of DCs in priming T cells activation.These results provide an interesting novel insight into the potential mechanism by which CK contribute to the restoration of immunoregulation in autoimmune conditions.

ginsenoside metabolite compound K;dendritic cells;T cells;collegen-induced arthritis

The project supported by National Nature Science Foundation of China(81503084,81330081,31200675,and 81173075)

Wei WEI,Tel:86-551-516-1208,E-mail:wwei@ahmu.edu.cn