红富士苹果芽变优系的SSAP分析

王江波,吴翠云,张锐

摘要：采用SSAP分子标记技术对田间自然产生的红富士苹果（Malus domestica）新芽变材料及其对照进行了遗传鉴定。结果表明，一个376 bp DNA片段为芽变材料特异性片段，其序列与苹果查尔酮合成酶（CHS）基因启动子同源性达90%，表明芽变材料是遗传物质发生改变的稳定变异，为进一步研究芽变材料发生性状变异的分子机理奠定基础。

关键词：苹果（Malus domestica）；芽变；SSAP；分析

中图分类号：Q789；S661.1 文献标识码：A 文章编号：0439-8114（2013）05-1181-04

SSAP Analysis of Red Fuji Apple Bud Mutation

WANG Jiang-bo1a，2，WU Cui-yun1a，2，ZHANG Rui1b，2

（a.College of Plant Science；b. College of Life Science，Tarim University， Alar 843300， Xinjiang， China；2.Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin， Xinjiang Production and Construction Corps， Alar 843300， Xinjiang， China）

Abstract： Fuji apple bud mutation and the wild type were analyzed by SSAP. The results showed that a DNA fragment of 376 bp in length was specific fragment of Fuji apple bud mutation， and it was up to 90% homology with the promoter of chalcone synthase（CHS） gene. It indicated that Fuji bud mutation was stable mutation of the genetic material changes， and laid a basis for further research on the molecular mechanism of bud mutation.

Key words： apple（Malus domestica）； bud mutation； SSAP； analysis