IR780/Gemcitabine-conjugated metal-phenolic network enhanced photodynamic cancer therapy

Songtao Zhou ,Hao Tian ,Jie Yan ,Zhan Zhang ,Guohao Wang ,Xinying Yu ,Wei Sang,Bei Li,Greta S.P.Mok,Jie Song,Yunlu Dai,∗∗

a Cancer Center and Institute of Translational Medicine,Faculty of Health Sciences,University of Macau,Macau 999078,China

b MoE Frontiers Science Center for Precision Oncology,University of Macau,Macau 999078,China

c Institute of Basic Medicine and Cancer (IBMC),Chinese Academy of Sciences,The Cancer Hospital of the University of Chinese Academy of Sciences,Hangzhou 310022,China

d Biomedical Imaging Laboratory (BIG),Department of Electrical and Computer Engineering,Faculty of Science and Technology,University of Macau,Macau 999078,China

Keywords: Metal phenolic network IR780 Photodynamic therapy Breast cancer Gemcitabine

ABSTRACT Photodynamic therapy (PDT) is a clinically approved cancer treatment that uses energy of light to generate active substances that cause damage to the cancer.Photosensitizers are employed to absorb light and generate toxic reactive oxygen species (ROS) to damage biomolecules like DNA.At the same time,some chemotherapy drugs like nucleotide analogues can provide mechanism-guided promotion in the treatment efficacy of PDT.However,the photosensitizer and chemotherapy drugs used in PDT is usually organic molecules,which suffers from bad solubility,fast clearance,and acute toxicity.To achieve targeted treatment,a reasonable delivery system is necessary.Therefore,we reported a metal-phenolic network where IR780 and gemcitabine were coupled chemically to overcome these shortcomings.The enhanced PDT effects can be realized by the promoted cell death both in vitro and in vivo.Moreover,the synergistic therapy also induced T-cell mediated anti-tumor immune response,which was significant for the inhibition of distant tumor growth.This work expanded the biomedical application of metal-phenolic materials and contribute to the wider application of photodynamic cancer therapy.

Photodynamic therapy (PDT) is a promising therapeutic procedure approved in clinic in the field of oncology [1–3].Various types of photosensitizers have been developed for PDT,including porphyrins,chlorins,and cyanine derivatives.Followed by irradiation,the light of specific wavelength can activate these photosensitizers to produce reactive oxygen species (ROS),which induce cell apoptosisviadamaging biology molecules including lipid and DNA.To amplify the PDT efficacy,combination of chemotherapeutic drugs is quite effective.For example,nucleotide analogues have been used clinically for decades to induce DNA damage in cancer cells which provide mechanism-guided promotion in the treatment efficacy of PDT by inhibiting DNA repair and replication [4–10].

However,both photosensitizers and nucleotide analogues suffer from poor solubility and limited biocompatibility which greatly limit their applications.To overcome these limitations and achieve the best therapeutic effect,different strategy such as PEGylation has been developed to improve water solubility and prolong cycle time of hydrophobic drug [11–16].But only the chemical conjugation cannot achieve targeted delivery to tumor sites.Nowadays,some nano systems have shown great potential in cancer diagnosis and treatment with their advanced tumor accumulation and abundant unique functions [17,18].Various metal containing nano delivery systems have shown potential for further application [19,20].Therefore,it is promising to employ nano materials for targeted tumor delivery [21].

Metal-phenol network (MPN) is a newly developed nanovehicle which form stable nanoparticlesviahydrophobic interaction and phenol-metal coordination [18].Besides,the pHresponsive coordination also allows the degradation of nanoparticles and release cargoes in acidic tumor microenvironment[22–27].Our group has been working on expanding application of MPN materials on cancer theragnostics and realized effective suppression for various types of tumors in different therapies [28–40].

In this work,we exploited IR780 as PDT agent [41] that induced significant DNA damages when exposed to laser irradiation.And gemcitabine was introduced to enhance the therapeutic effects of PDT by inhibiting DNA repair.Both IR780 and gemcitabine were PEGylated into phenolic polymer and form NP in presence of ferric ions (Scheme 1).The experimentsin vitroandin vivocan confirm the enhanced PDT performance.Furthermore,the synergistic therapy also induced anti-tumor immune response,which inhibited the growth of distant tumor [42,43].

Scheme 1.Schematic illustration about the design of the metal-phenolic network.The IR780 and gemcitabine were PEGylated to form metal phenolic network (MPN)to overcome their inherent defects.And the synergistic therapy enhanced photodynamic therapy by promoting cell apoptosis and facilitating T-cell mediated antitumor immune response.

Based on our previous work,we designed and synthesized two amphiphilic polymers (PEG-IR780 &PEG-Gem) to overcome these disadvantages of IR780 and gemcitabine [28,44].The chemical structure and synthesis route has been shown Figs.S1 and S2 (Supporting information),the polyethylene glycol (PEG5000) was chosen as the hydrophilic chain.To synthesize the polymers,the polymers containing free amino groups orN-hydroxysuccinimide (NHS)groups at the hydrophobic alkane chain were obtained through reversible addition fragmentation chain transfer (RAFT) reaction.Subsequently,polyphenol,IR780 and gemcitabine were decorated into the polymers,respectively.The final products were characterized and quantified by UV and1H NMR (Figs.S3-S5 in Supporting information).The number of gemcitabine molecules on PEG-Gem was about 12–13,quantified by analyzing the characteristic peak of gemcitabine at 269 nm (Figs.S3 and S4).The number of IR780 and catechol on PEG-IR780 was about 8 and 6 respectively quantified by1H NMR (Fig.S5).The conjugation of gemcitabine is reversible in acidic environment according to previous work,endowing it pHresponsive ability (Fig.S6a in Supporting information) [45].Therefore,we measured the drug release rate of gemcitabine from PEGGem in different pH conditions.It increased significantly when pH was reduced from 7.4 to 6.5 (Fig.S6b in Supporting information).

For the preparation of NP,two polymers at the same concentration were added to Fe3+aqueous solution under ultrasonic condition to trigger the rapid assembling of NPviahydrophobic interaction and metal-phenolic coordination (Fig.1A).The size and morphology were determined by DLS and TEM,where the average diameter of particles was 76.7 nm (Figs.1B and C).The surface charge of the NP was also determined,where the zeta potential of the NP was 14.9 mV (Fig.S7 in Supporting information).Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify ferric ions in NP,where the mass proportion of Fe3+in NP was 0.68%.To further confirm the stability,we monitored the size of the particles in 7 days and found the particle was quite stable in one week after assembling (Fig.S8 in Supporting information).Furthermore,we found the modified IR780 showed a blue shift in absorption (640 nm) and the fluorescence at about 750 nm (Fig.1D).Then we evaluated the PDT effect of the NP.1,3-Diphenylisobenzofuran (DPBF) was a specific ROS probe,which was chosen for the detection of ROS production.the result showed that the nanoparticles could amplify the ROS generation significantly under the 660 nm laser irradiation (Fig.1E).

Fig.1.Design and characterization of NP.(A) Scheme of NP.(B) TEM images of NP.Scale bar: external 500 nm and internal 200 nm.(C) Size distribution of NP determined DLS.(D) Absorption (blue) and emission (red) of NP.(E) ROS generation of the NP under 660 nm laser irradiation.(+): with 660 nm laser,(-): without 660 nm laser,1 W/cm2.Data are presented as mean values ± SD (n=3).

Mice breast cancer cell 4T1 was chosen for therapeutic experiments.Firstly,the red fluorescence from IR780 was exploited to evaluate the cellular uptake of NP.The change of fluorescence intensity at different time points (0,2,4,8,12,24 h) showed the cellular uptake behavior increased over time and it was found that 24 h is enough for most NP being administrated (Fig.2A).Intracellular ROS production was evaluated by the fluorescence probe H2DCFDA which reacted with singlet oxygen and generated a green fluorophore.Due to the self-fluorescence of PEGIR780,the intracellular biodistribution of the NP could also been traced by red fluorescence (Fig.2B).The obvious increase in green fluorescence under irradiation indicated the ROS generation.The co-location of green and red fluorescence also demonstrated the spatiotemporal consistency of the NP and produced ROS.To confirm the amplified DNA damage,antibody againstγ-H2AX was used to detect DNA double strand breaks.Treated with NP and 660 nm laser,a stronger red fluorescence was generated and merged with the blue fluorescence from DAPI to produce a purple signal (Fig.2C).Methyl thiazolyl tetrazolium (MTT) assay was conducted to evaluate the cytotoxicity of various treatments(Fig.2D).Compared with laser irradiation or gemcitabine alone,NP combined with laser treatment got the best anti-cancer effects,which demonstrated the synergistic treatment can enhance PDT.Annexin V/PI apoptosis staining was performed to further confirm the enhanced PDT of NP (Fig.2E).Combining NP and irradiation treatment,total apoptosis ratios obviously increased from 4.99% to 14.09% compare with control group.

Encouraged by the good performance of the NPin vitro,further experimentsin vivowere conducted.All animal studies were approved by the University of Macau Animal Ethics Committee following the standard protocol (UMARE-030–2018).As mentioned before,PEGylation tend to prolong the residence time and facilitate tumor accumulation.The fluorescence from IR780 was used to monitor the biodistribution of NP.In this experiments,4T1 tumorbearing mice model was chosen.After the intravenous injection of NP,thein vivofluorescent imaging was conducted at different time points (1,4,8,12,24 h).Initially,the fluorescence in tumor site increased and then gradually decrease,peaked at 4–8 h (Fig.3A).And the total fluorescent intensity was quantified (Fig.3B).Moreover,the fluorescence in tumor site was still remarkable at 24 h.Then the tumors and main organs (heart,liver,spleen,lung,kidney)were collected forex vivofluorescent imaging.The corresponding quantification results further confirm the high tumor accumulation(Figs.3C and D).

Fig.3.In vivo experiments.(A) In vivo fluorescent images at different time points.(B) Quantitative analysis of the total fluorescence in tumor areas (n=3).(C) Ex vivo fluorescent images of major organs at 24 h post-injection.(D) Quantitative analysis of ex vivo fluorescence at 24 h post-injection.(E) Anti-tumor experiment procedure (dose of drug was quantified by polymer:100 μg/mice).(F) Primary tumor volume.(G) Distant tumor volume.(H) Body weight of mice.G1: PBS,G2: PEG-Gem,G3: PEG-IR780 (-),G4: PEG-IR780 (+),G5: NP (-),G6: NP (+),where (+) represent the 660 nm laser treatment 6 h after injection.Data are presented as mean values ± SD (n=4).

Fig.4.Immune evocation.Flow cytometric and quantitative analysis of CD4+ T cells (A) and CD8+ T cells (B) in distant tumors.Quantification data of CD4+ T cells (C) and CD8+ T cells (D) in primary and distant tumors.G1: PBS,G2: PEG-Gem,G3: PEG-IR780 (-),G4: PEG-IR780 (+),G5: NP (-),G6: NP (+),where (+) represent the 660 nm laser treatment 6 h after injection.Data were presented as mean values ± SD (n=3).

Then we evaluated thein vivoantitumor effects of the NP on bilateral subcutaneous tumor-bearing mice model,and the procedure is shown in Fig.3E.When the primary tumor volume reached 200 mm3,all mice were randomly divided into six groups: G1: PBS,G2: PEG-Gem,G3: PEG-IR780 (-),G4: PEG-IR780 (+),G5: NP (-),G6: NP (+),where (+) represents the 660 nm laser treatment at 6 h after injection.To avoid potential photothermal therapy (PTT)effect,we took 30 s intervals for cooling down after every 30 s laser treatment.The volume of tumors and body weight were monitored and recorded every two days until all mice were sacrificed on day 15.The main organs,serum,and tumors were collected for further analysis.Compared with the PBS group,the two polymers,PEGGem and PEG-IR780 alone only had a slight tumor suppressing effect (Fig.3F).And as expected,the NP treated mice supported with irradiation got the best tumor suppression for both primary and distant tumor (Figs.3F and G),which indicated the effective synergy of NP and PDT.We assume the therapy effects for distant tumor was achievedviathe immune response.

To evaluate the biomedical safety of the NP,we traced the body weights of mice during treatments,analyzed biochemical parameters of serum,and evaluate the histomorphology changes of major organs of miceviaH&E staining.The body weight of the mice under different treatment showed no obvious difference (Fig.3H).After being sacrificed,mice serum was collected and blood biochemical parameters was evaluated (Fig.S9 in Supporting information),including lactate dehydrogenase (LDH),creatine kinase (CK),glutamic oxaloacetic transaminase (GOT),glutamic pyruvic transaminase (GPT),blood urea nitrogen (BUN),and creatinine (CR).Those paraments did not show significant difference among groups that indicate this NP has no obvious side effects on the mice body.H&E staining of major organs demonstrated that the histomorphology of the heart,liver,spleen,lung,and kidney was not affected noticeably (Fig.S10 in Supporting information).4T1 cells induced tumor is known to be highly migratory,so we also evaluated the lung metastasis to see if this treatment could also suppress tumor migration.Bouin’s fixative solution was used to fix the collected lungs after treatments.By observing the metastasis nodules on lung surface (Fig.S11 in Supporting information).We found this treatment can also slow the lung metastasis process.

We hypothesized that the inhibitory effect on distant tumor growth was mainly due to the antitumor immune response.As a representative,we investigated the T cells activation and filtration in tumor.Helper T cells (CD3+CD4+) and cytotoxic T cells (CD3+CD8+) in primary and distant tumors was evaluated by flow cytometry.The total percent of CD4+helper T cells and CD8+cytotoxic cells in CD3+cells increased (Figs.4A and C,Fig.S12 in Supporting information).These improvements could be more obvious in distant tumor where CD4+cell percent was increase from 10.4% to 30.7% and CD8+cells also increase from 6.77% to 19.9% (Figs.4B and D,Fig.S13 in Supporting information).Moreover,relative high level of IFN-γand TNF-αin tumor may imply an improved immune environment after treatments (Fig.S14 in Supporting information).These results indicate this NP will facilitate the T-cell mediated immune response in tumor.

To make a summary,we decorated IR780 and gemcitabine into phenolic polymer to form NP,which overcome the poor solubility and increase the cycle time of the two hydrophobic drugs.The MPN delivery system also endowed it good tumor accumulation and pH-responsive drug release,which increased the selectivity of the drugs and reduce the toxicity to normal tissues.The DNA was damaged by PDT,and DNA repair and replication was inhibited by gemcitabine thus aggravate the damage and suppressed tumor growth.Furthermore,this NP could also evoke immune response and suppress distant tumor growth.We highlight the advantages of the MPN delivery system and expand its application where chemical conjugation could be used to improve the biocompatibility and effects of hydrophobic drugs.Metal-containing nanodrugs have been widely used in cancer diagnosis and treatment.We combined the advantages of different therapeutic agents with MPN delivery system,which could broaden our acknowledge of nano delivery system for cancer and contribute to the wider application of metal-containing nanodrugs and photodynamic cancer therapy.

Declaration of competing interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC,Nos.32171318,32222090 and 32101069),the Faculty of Health Sciences,University of Macau,the Multi-Year Research Grant (MYRG) of University of Macau (No.MYRG2022–00011-FHS),the Science and Technology Development Fund,Macau SAR (Nos.0103/2021/A and 0002/2021/AKP),Shenzhen Science and Technology Innovation Commission,Shenzhen-Hong Kong-Macau Science and Technology Plan C (No.SGDX20201103093600004),and Dr.Stanley Ho Medical Development Foundation (No.SHMDFOIRFS/2022/002) The authors appreciate the assistance and support from the Proteomics,Metabolomics,and Drug Development Core,Animal Research Core,and Biological Imaging and Stem Cell Core in the Faculty of Health Sciences,University of Macau.

Supplementary materials

Supplementary material associated with this article can be found,in the online version,at doi:10.1016/j.cclet.2023.108312.