Salt-tolerant Microbiota Enhancing Contaminants Removal from Mariculture Wastewater Containing Sulfamethoxazole in an A/O-MBBR

ZHENG Bingbing, PANG Wenhui, ZHAO Yangguo, 2), *, GUO Liang, 2), GAO Mengchun, 2), JIN Chunji, 2), and SHE Zonglian, 2)

Salt-tolerant Microbiota Enhancing Contaminants Removal from Mariculture Wastewater Containing Sulfamethoxazole in an A/O-MBBR

ZHENG Bingbing1), PANG Wenhui1), ZHAO Yangguo1), 2), *, GUO Liang1), 2), GAO Mengchun1), 2), JIN Chunji1), 2), and SHE Zonglian1), 2)

1) Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering (MEGE), College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100,China 2) Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China

The wide application of antibiotics in aquaculture requires an efficient treatment of the wastewater before discharging it into the environment. During the wastewater treatment, the influence of antibiotics on the performance of bioreactor should be well revealed due to their toxicity to the functional microbial community. In this study, the effect of feeding 10-30mgL−1sulfamethoxazole (SMX) in influent on the performance of an anoxic/oxic-moving bed biofilm reactor (A/O-MBBR) treating mariculture wastewater and the responding change of biofilm microbial communities was investigated. The COD average removal rate remained at 94.61%-97.34% with the dosage of SMX. Compared with that, the nitrifying removals of NH4+-N and NO2−-N were violently inhibited by 30mgL−1SMX and denitrifying removal of the NO3−-N decreased obviously with 20mgL−1or more SMX. The microbial community in the successful startup bioreactor was relatively abundant, while the diversity of microbial community decreased with the increase of feeding SMX. The salt-tolerant and SMX-resistant genera,,andwere gradually enriched and finally played a vital role in converting COD and recycling nitrogen and sulfur. Hence, the present A/O-MBBR reactor with the salt-tolerant functional microbiota achieved efficient removal of pollutants in the presence of low concentration (.., 10mgL−1) SMX.

sulfamethoxazole; mariculture wastewater; inorganic nitrogen; microbial community; A/O-MBBR

1 Introduction

Nowadays, the requirement for high-quality protein is increasing. Seafood, as a protein-rich product, becomes more popular in our menu (Liu., 2017; Mo., 2017). To meet the increasing demand for seafood, more aquaculture or mariculture systems for culturing the fish either in freshwater or seawater, have to be built since there has been a gradual decrease of wild fishery resources. Thus, large amounts of wastewater containing suspended solids, nitrogen compounds, organic matter and bait are produced. Till now, the mariculture wastewater is usually discharged directly into nearby river of offshore without treatment due to high salinity, low carbon/nitrogen ratio (Zheng., 2016). China is the largest producer and exporter of seafood and contributes to 61.70% of the global aquaculture production (Liu., 2017). However, the wastewater from aquaculture is often in positive proportion to the production. As such, the increased discharge of mariculture wastewater and the pollution has drawn more attention. The efficient treatment for the high-salt wastewater containing high total nitrogen is urgently required. In addition, China is isussing more strictly aquaculture wastewater discharging standards, where more efficient wastewater treatment techniques and higher quality effluent are fundamental.

Infectious diseases are recognized to be one of the most frequent disasters in aquaculture (Lagana., 2011). Hence, antimicrobial compounds are often used in the baits to prevent fish diseases (Stolker and Brinkman, 2005; Saxena., 2018). The intensive farming practices in China are commonly accompanied by outbreaks of infectious diseases (Mo., 2017). Antibiotics are widely used in aquaculture to treat infections caused by a variety of bacterial pathogens, and even some prohibited antibiotics have also been found in recent years (Liu., 2017). As the largest producer and user of antibiotics, China consumed 162000 tons of antibiotics in 2013 (Zhang., 2015). Liu. (2017) found that the types of antibiotics in mariculture wastewater were more than those in freshwater aquaculture wastewater, indicating that the pollution of mariculture wastewater was even more serious. Antibiotics can inhibit the growth of pathogens, but they can also kill the beneficial microbes, which will finally disturb the balance of the ecosystem. The risks of antibiotics to human health are also not to be neglected due to adverse drug reactions and the potential epidemic of antibiotic resistance pathogens caused by selective pressure on bacteria (Xiong., 2015; Sun., 2016).

Long-term overuse of antibiotics resulted in rapid transfer of antibiotic resistance genes (ARGs) in the environment (Wang., 2018). In addition, Li. (2017) found that antibiotics reduced the diversity of microbial community and changed the bacterial community structure. There also many studies showed that antibiotics could block the nitrification and denitrification processes in wastewater treatment (Zheng., 2016; Li., 2017), which will bring more barriers to the biological processes. Hence, removal of the antibiotics by biological processes from the wastewater is a big challenge.

The biofilm-based wastewater treatment processes had more advantages over other methods, the advantages include higher biomass, longer sludge retention times, stronger resistance to shock loading, easier to separate sludge from water. In addition, the biofilm could provide more diverse ecological niches for different functional microbes to survive, so the microbes could cooperate with each other to completely degrade complex chemicals (William., 2013). Most importantly, once the microbes form biofilms, the biofilms presented higher resistance to antimicrobial agents (Wolcott., 2010), which made it possible to use the biofilm-based technique to treat the antibiotics-containing wastewater. Therefore, in this study, the anoxic/oxic (A/O) and mobile bed biofilm reactor (MBBR) were combined to treat mariculture wastewater containing high-nitrogen and antibiotics.

Sulfamethoxazole (SMX) is one of most commonly used sulfonamides antibiotics in mariculture. The SMX dose generally ranged from 100 to 200mgkg−1of fish body weight and SMX concentration in culturing pond was sometimes up to 25mgL−1(Liu., 2017). Therefore, this study adopted 10-30mgL−1SMX to explore the effect of the antibiotics on the performance of A/O- MBBR with salt-tolerant microbiota. Meanwhile, the response and change of microbial community were investigated and compared horizontally.

2 Materials and Methods

2.1 Bioreactor

The integrated wastewater treatment bioreactor, A/O- MBBR was used in this study (Fig.1). The bioreactor was consisting of three parts, that is, anoxic zone, oxic zone and sedimentary zone, and its total effective volume was 11.88L. Firstly, the wastewater was pumped into the anoxic zone, where a mixing blender was installed. Then, the water flowed into the oxic zone, where the aerator device was set to provide enough oxygen. In order to sufficiently remove the nitrate in the water, a reflux system was built to pump the water back to the anoxic zone. About one third volume of carriers with a diameter of 25 mm and an effective surface area of more than 500m2m−3, were filled in the anoxic and oxic zones. The sedimentary zone with a volume of 0.84L followed the oxic zone.

Fig.1 The schematic diagram of the A/O-MBBR process. 1, Artificial wastewater tank; 2, Influent pump; 3, Anoxic zone; 4, Blender; 5, Oxic zone; 6, Reflux pump; 7, Aerating pump; 8, Microporous aerator; 9, Sedimentary zone; 10, Effluent; 11, Carrier (right top picture shows the carrier covered with biofilm).

2.2 Startup and Operation of the Reactor

The seed sludge used in this study was taken from the secondary sedimentation tank of Tuandao wastewater treatment plant, China. In order to form the biofilm on the carriers, the batch mode operation was adopted at the startup stage of bioreactor in the first four weeks. One batch cycle contained 18h of operation and 4h of idling. During bioreactor operation, the artificial mariculture wastewater was sequentially pumped into the anoxic and oxic zones, where the water was completely mixed with activated sludge and carriers. The blender in the anoxic zone kept stirring during the operation; the microporous aerator at the bottom of the oxic zone kept the dissolved oxygen at about 5.00mgL−1. After operation, all devices stopped working and kept stationary for 4h and then the water was discharged. From the fifth week, the bioreactor was changed to continuous operating mode, where the Hydraulic Retention Time (HRT) was 8 h, and the reflux ratio was 100%. The outside temperature was 17-20℃.

The artificial mariculture wastewater was prepared according to the literature (Zheng., 2016), and the salinity was adjusted by adding the concentrated seawater (Yanrui New Materials, Qingdao, China). During the batch operation, the salinity of wastewater was initially set at 5‰ and it was then improved by 1‰ daily and finally to 30‰. As the bioreactor came into continuous mode, the salinity was kept stably at 30‰. The artificial wastewater composition contains (mgL−1): NH4Cl 18.15, NaNO352.15, NaNO23.54, KH2PO415.00, CH3COONa190.00, Na2CO3130.00, pH 6.50-7.50. The ratio of COD to total nitrogen was about 10.50, where the total inorganic nitrogen and COD were equal to 14.00 and 148.00mgL−1.

Three A/O-MBBR bioreactors (named A, B and C) were operated synchronously to investigate the influence of SMX on the performance. After successful startup, 10, 20 and 30mgL−1SMX (Aladdin, Shanghai, China) was separately fed into the influents of three A/O-MBBR bioreactors A, B and C for eight consecutive days. The performance of bioreactors was monitored by analyzing the influent, as well as the effluent parameters. The biofilms attached to the carriers were obtained to reveal the changes of microbial community.

2.3 Analysis Methods

The water quality parameters, including COD, NH4+-N, NO3−-N and NO2−-N, in the influent and effluent were examined daily referring to Chinese NEPA standard me- thods (Chinese NEPA, 2002).

The seeding sludge (Sl), biofilm samples in anoxic zone (AS0) and the oxic zone (OS0) at successful startup of bioreactors, and the biofilm samples in anoxic zone (AS10, AS20, AS30for bioreactor A, B and C) and the oxic zone (OS10, OS20, OS30for bioreactor A, B and C) at the end of operation were taken to analyze the diversity of microbial community by using 16S rRNA gene based high-through- put sequencing.

The total DNA of the sludge and biofilm samples was extracted by the Soil DNA Extraction Kit (Mobio, CA) according to manufacturer’s instructions.Partial 16S rRNA gene based high-throughput sequencing was used to determine the diversity and composition of the bacteri- al communities in each sample. PCR amplifications were conducted in triplicate with the primer set 515F (5’- GTGCCAGCAGCCGCGGTAA-3’) and 806R (5’-GGA CTACCAGGGTATCTAAT-3’) targeting the V4 region of the bacterial 16S rRNA gene. The reverse primer contained a 6bp error-correcting barcode unique to each sample. PCR was performed following the protocol described previously (Gao., 2014). Afterwards, the purified PCR products weresubjected to high throughput sequencing on an Illumina MiSeq platform by Novogene (Tianjin, China). QIIME software package (http://qiime. org/) and UPARSE pipeline (http://drive5.com/uparse/) were used to analyze the reads and pick operational taxo- nomic units (OTUs). Sequencing results were filtered by splicing, and sequences with 97% or more similarities were classified into same OTU. The Rarefaction plots, Shannon index, Principal Component Analysis (PCA), and species composition and relative abundance of microbial community were analyzed by the methods as described in literature (Gao., 2014). All sequences obtained in this study were deposited in the MG-RAST database under the accession Nos. mgs751504-mgs751512.

3 Results and Discussion

3.1 Effect of SMX on the Performance of the A/O- MBBR

The bioreactor efficiency at successful startup is listed in Table 1. The corresponding changes of bioreactors performance with SMX dosage are shown as Fig.2. Almost all COD was removed as the bioreactors started up. When 10, 20 and 30mgL−1of SMX were added in influent, the average removal rates of COD reached 97.34%, 94.61% and 95.53% respectively, corresponding average COD 4.01, 8.02 and 6.68mgL−1in effluent, which showed that the COD removal was not heavily repressed by the high concentration of SMX. Similarly, Du. (2018) indicated that tetracycline exposure had little effect (>0.05) on the efficiency of COD removal during the whole period of lab-scale A-O systems.

Table 1 The performance of the bioreactors as they started up

With the influent containing 0, 10, 20 and 30mgL−1SMX, the average removal rates of NH4+-N in bioreactors decreased from 83.69% to 76.11%, 77.50% and 53.02% respectively, resulting in 1.13, 1.06 and 2.22mgL−1NH4+- N in effluent. Interestingly, the average removal rates of NO2−-N even increased from 81.67% to 85.08%, 89.62% due to feeding of 10, 20mgL−1SMX; while it quickly went down to 57.80% due to feeding of 30mgL−1SMX. Tong. (2019) also found that low centration of ofloxacin increased the NH4-N removal efficiency from 72.62% to 80.66–82.13%. But the mechanism involved in should be further elucidated. The above results showed that the nitrifying process performed well as feeding 20mgL−1or less SMX. 30mgL−1SMX resulted in a significant decline for the removal of ammonia and nitrite, which finally led to accumulations of ammonia and nitrite.

The average removal rates for NO3−-N were 78.61%, 78.84%, 65.23% and 56.18% for the influent containing 0, 10, 20 and 30mgL−1SMX. Hence, compared with performance of bioreactors without SMX feed, 20-30mgL−1SMX presented an obvious inhibition on the denitrification. The denitrifying process revealed more sensitive to the SMX feeds than the nitrifying process and COD removal, which accorded well with the results of Zhu. (2017). They concluded that denitrification was more susceptible than nitrification and carbon oxidization processes under SMX stress.

The nitrogen removal efficiency presented a downward trend due to increased feeding concentrations of SMX. The reactor performance was then finally damaged in the removing the nitrogen when feeding 30mgL−1SMX. Zheng. (2016)reported that the bioreactor fed the mariculture wastewater containing oxytetracycline from 0 to 12mgL−1led to obvious decrease of the removal rates of NH4+-N and COD. Similarly, it was found that the removal rates of COD and NH4+-N decreased and the effluent NO2−-N and NO3−-N increased with the elevation of feeding florfenicol (Gao., 2018). Researchers demonstrated that the presence of antibiotics (such as erythromycin, tetracycline and SMX) impacted the nitrogen cycling and organic matter degradation by inhibiting the activity of key enzymes in different wastewater biological treatment processes (Cetecioglu., 2013; Katipoglu-Yazan., 2015). Comparing the control and the previous results, feeding 10mgL−1SMX presented almost no impact on the performance of mariculture wastewater by A/O-MBBR in this study. Moreover, 10-20mgL−1SMX even enhanced the removal of NO2−-N. As revealed by present study, 10-20mgL−1SMX feeds lightly inhibit the ammonia oxidation process and denitrification process, this would finally decrease the production of nitrite. Therefore, the part reason for increase of NO2−-N removal rate might be due to the decrease of production of NO2−-N. The removal rates of COD and nitrogen species responding differently to SMX feeds might be ascribed to that the different functional microbes revealed different sensitivity to the SMX and the biofilm in bioreactor provided diverse niches for the growth of microbes (Gui., 2017; Zhu., 2017).

Fig.2 Effects of different concentrations of SMX feeds on the removals of COD (a), NH4+-N (b), NO2−-N (c) and NO3−-N (d) in the A/O-MBBR.

3.2 Effects of SMX on the Diversity of Microbial Communities

The diversity of microbial community in the three reactors changed, especially after SMX dosage (Fig.3). SMX feeding decreased the diversity of microbial communities in both the anoxic and oxic zones, which indicated that largely microorganisms were washed out under the SMX press and some species with high resistance to SMX were obviously enriched. Interestingly, in the oxic zones, the bioreactor feeding with 30mgL−1SMX presented higher diversity than that of bioreactor with 20mgL−1SMX. When the SMX concentration in the influent was 10, 20, 30mgL−1, the number of microbial species in the anoxic zone decreased from 1014 (AS0) to 720 (AS10), 751 (AS20), 798 (AS30), and the corresponding diversity index decreased from 7.11 to 4.67, 3.95 and 3.98.Meanwhile, the number of microbial species in oxic zone decreased from 924 (OS0) to 724 (OS10), 545 (OS20), 540 (OS30), and the corresponding diversity index decreased from 6.64 to 5.05, 2.36 and 3.02.

The shared or unique OTUs for different microbial samples was analyzed and the results are shown in Fig.4. In the anoxic zones, the shared OTUs for the biofilm samples at the end of the fifth week (AS0) and the biofilm samples with 10, 20, 30mgL−1of SMX (AS10, AS20, AS30) were 364; while in the oxic zones, the shared OTUs was 324. As for the unique OTUs, the samples at the end of fifth week contained greater values, 227 OTUs for anoxic zone and 243 OTUs for the oxic zone. PCA for the microbial communities (Fig.5) showed that the microbial populations of seed sludge (SI) was quite different from the biofilms, which indicated that the enrichment by wastewater in bioreactor impacted heavily on the micro- bial composition. Similarly, compared with the AS0and OS0samples as the bioreactor successful startup, the SMX feeds largely changed the microbial composition in bio- films. The SMX feeds presented some different influence on the biofilms in anoxic and the oxic zones. The sample in the oxic zone under the 10mgL−1SMX dosage went to the adverse direction as compared with the samples under the 20, 30mgL−1SMX feeds. These results showed that nitrification process under the 10mgL−1SMX feeds was quite different with that of 20, 30mgL−1SMX feeds.

Fig.3 Rarefaction plots (a, b) and Shannon index (c, d) of microbial communities in seed sludge (SI) and biofilm samples from anoxic zone (AS0, AS10, AS20, AS30) and oxic zone (OS0, OS10, OS20, OS30).

Fig.4 Venn map of the shared and unique OTUs for biofilm samples from anoxic zone (AS0, AS10, AS20, AS30) (a) and oxic zone (OS0, OS10, OS20, OS30) (b) at different concentrations of SMX feeds.

Fig.5 Principal Component Analysis (PCA) of microbial communities in seed sludge (SI) and biofilm samples from anoxic zone (AS0, AS10, AS20, AS30) (a) and oxic zone (OS0, OS10, OS20, OS30) (b).

Fig.6 shows the relative abundance of microbial communities in phylum or class. The microbial community of seeding sludge (Sl) presented the highest abundance with 12 phyla, among whichheld the highest relative abundance. At the end of startup of the reactor, the relative abundance ofin samples increased to 18.02% and 20.16% in the anoxic and oxic zones with the improvement of salinity. At the same time, the relative abundance ofincreased from 9.27% to 25.97%, and that ofremained stable at about 8.30%. In addition,andgradually decreased slightly. Overall,was gradually enriched with salinity improvement during startup, which indicated that most of the microorganisms inwere salt-tolerant.

Fig.6 Microbial communities’ composition and relative abundance in seed sludge (SI) and biofilm samples from anoxic zone (AS0, AS10, AS20, AS30) and oxic zone (OS0, OS10, OS20, OS30).

In the anoxic zone of the reactor, with the increase of SMX concentration, the relative abundance ofin biofilm increased from 17.85% to 58.22%, 10.41% and 59.19%, respectively.was also enriched slightly. On the contrary,andgradually reduced.tended to be gradually enriched.

In the oxic zone of the reactor, with the increase of SMX concentration, the relative abundance changes of microorganisms at the phylum level were similar with those in the anoxic zone. The relative abundance ofin biofilm increased from 20.96% to 49.79%, 83.31% and 69.29%, respectively.,,andgradually decreased, which was different from previous report by Zheng. (2016), where they found out that theincreased largely with the rise of the concentration of norfloxacin. This might be due to the difference of antibiotics, as different antibiotics might present unique inhibition on some bacteria. In general,in biofilm tended to be gradually enriched with the treatment of artificial mariculture wastewater containing SMX.

Previous research (Katipoglu-Yazan., 2015) show- ed that long-term SMX exposure gradually reduced the gene expression of nitrifying bacteria and impaired cell activity. Meanwhile, partial degradation of SMX by heterotrophic bacteria was observed. Based on the changes in the operation efficiency of the comprehensive reactor and the number of same and endemic OTU of microorganisms, it was speculated that with the increase of the concentration of SMX, some heterotrophic bacteria capable of degrading organic matter were enriched in the anoxic zone of the reactor. Therefore, the removal of COD in the reactor remained acceptable. However, some ammonia-oxidizing, nitrifying and denitrifying bacteria might reduce, consequently the removal rates of NH4+-N, NO2−-N and NO3−-N greatly decreased.

3.3 Salt-Tolerant Microbiota Contributing to Contaminants Removal

As for the oxic zone of bioreactor when it started up, the generaands were largely enriched in aerobic biofilm (OS0) and theyreached to 9.27% and 6.24% of total bacteria (Table 2). Some other salt- tolerant bacteria, such as,,,,,,,were also improved to above 1% of total bacteria, which indicated that high salt in the seawater played a key role in enriching the specific bacteria. The typical nitrifierpresented a little increase to 0.67%. However, when the SMX was added, the profiles of dominant bacteria chang- ed significantly. Almost all above mentioned enriched bacteria declined in relative abundance except thes, which was further improved to 40.72% under 10mgL−1SMX, 76.17% under 20mgL−1SMX, and 63.24% under 30mgL−1SMX. The genera,,jumped to 5.01%, 2.55% and 6.70% in abundance as the reactor feeding with 10mgL−1SMX, but they decreased obviously as feeding with 20mgL−1and 30mgL−1.

Similar changes of microbial community in anoxic zone took place as the bioreactor started up (biofilm sample AS0). Compared with the sludge, the genera,,,,,,,,were largely enriched by high salt in biofilm sample AS0. However, most of the genera, except the, their abundance decreased with the increasing feed of SMX. The relative abundance of generaandalso presented an increase with the sudden feed of SMX which might suggest they were resistant to a low concentration of SMX (Zheng., 2016).

Table 2 Changes in relative abundance of the microbial communities in genus level

Note: Only the genera with relative abundance higher than 1% are shown.

Undoubtedly,is a very important genus in the bioreactor due to its high abundance, from 1.63% to 53.52%, throughout the operation. Some ofwere considered as the pathogens with resistance to many antibiotics (Kabeya., 2004). However, we further retrieved thesequences in NCBI nucleotide database and found that the most similar isolates (> 99.90%) werethe Candidatussp. CpA_a5 (Genbank Accession No: FN397893) from anoxic sub- surface mud breccia of the Capt Arutyunov mud volcanoa andDSM 7299 (CP001999) from the roots of cordgrass growing in salty marshes at the East coast of Canada. Compared with many otherspecies which are associated with warmlooded anmimals and tend to pathogenic,is micro- aerophilic, chemoorganotrophic, halo- tolerant, antibiotics-resistant and symbiotic bacterium with the ablity of fixing nitrogen and reducing nitrate in a marine envir- onment (Mcclung., 1983). In addition, manyspecies were also one of the most commonly found groups in wastewater (Guo., 2019; Pishgar., 2019) and they were commonly identified as the key potential denitrifier under the anoxic condition (Pishgar., 2019). Considering the bioreactor performance and corresponding abundance of, our results suggested this genus might play an important role in removing nitrogen in the high antibiotics and salinity wastewater treatment system.

, as an important driver of nitrogen and sulfur cycling, was largely enriched throughout the bioreactor operation. It is amazing that its relative abundance reached to 76.17% in the oxic zone as the bioreactor feeding 20mgL−1SMX. Many reports showed this group took part in the nitrogen and sulfur biogeochemical cycle and were responsible for the formation of granular sludge, sludge bulking and presented strong resistance to environmental pressure, such as the antibiotics (van den Akker., 2015; Du., 2019). Howarth. (1999) found out thatutilized different carbon sources to remove nitrite or nitrate in sewage treatment systems. Du. (2019) concluded that the abundance ofsignificantly increased from 3.46% to 24.09% and sludge bulking was accelerated by feedingthe 50mgL−1tetracycline and 10mgL−1SMX. Hence, the high abundance of, even not affected by SMX, may be the direct reason that the A/O-MBBR reactor presented acceptable removal efficiency on nitrogen in the treatment of aquaculture wastewater containing SMX.

was gradually enriched in anoxic zone by feeding the SMX. Previous research showed that they were salt tolerance, gram-negative, obligate anaerobic sulfur-reducing bacteria and exhibited complete oxidation of a wide range of electron donors (.., dicarboxylic acids and amino acids) linked to the reduction of sulfur (Liesackand Finster, 1994). However, till now, no results indicated that this genus could live or work under the antibiotics press. Hence, the presence ofmight contribute to the carbon and sulfur cycling.

, as one of important nitrifier, presented a high abundance (2.55%) in the biofilm of oxic zone with 10mgL−1SMX. They obtain energy from the oxidation of NH4+-N to NO2−-N. Silyn-Roberts and Lewis (2001) reported thatpresence rapidly increased as high as 7% of the total bacterial populations with the elevated ammonium concentrations. Kang. (2018) found thatcontained the genes capable of degrading SMX. Therefore, this genus was SMX-resistant and accelerated the oxidization of ammonia, which consequently enhanced the performance of the bioreactor.

4 Conclusions

The feeding of 10-30mgL−1SMX in bioreactor presented no negative impact on the COD removal. Nitrifying of NH4+-N and NO2−-N demonstrated more tolerant to increasing SMX than denitrifying of NO3−-N. The dosage of SMX decreased the diversity of biofilm microbial communities, salt-tolerant,,andwere extremely enriched. Combined the performance of bioreactor and their abundance, the cooperation of these bacteria played the fundamental role in COD removal, and nitrogen nitrification and denitrification. Finally, the process achieved the purpose of pollutants removal in the presence of low concentration of SMX.

Acknowledgements

We thank Dr. Progress Mapindu in Ocean University of China for his carefully revising our manuscript. This work was supported by the Fundamental Research Funds for the Central Universities of China (No. 201964004) and the National Natural Science Foundation of China (No. 41977315).

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. E-mail:ygzhao@ouc.edu.cn

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January 7, 2020;

February 14, 2020

(Edited by Ji Dechun)