Total Contents（Volume 11，2019）

COMMENTS

The recent advances and challenges in diagnosis and therapy of inherited metabolic disorders HAO Hu，XIAO Xin ………………………………………………………………………………………………………………………………（1）

Progress in the application of circulating tumor DNA detection in clinical diagnosis and treatment of colorectal cancer XIE Yingchao，ZHOU Chunlian，XU Weiwen …………………………………………………………………………………………………（73）

The role of circulating exosomal miRNAs in colorectal cancer and the challenges in clinical transformation Liu Xuejuan，Gan Haining，Zhang Limei，Xu Xuehu…………………………………………………………………………………………（157）

Advances of imaging quantitative techniques for liver fibrosis MA Mengjie，XIAO Zeyu，LIANG Jianye，LUO Liangping…………………………………………………………………………………（249）

Clinical application and significance of high-sensitivity hepatitis B virus-DNA detection WANG Jie，YU Guangxin，LU Fengmin………………………………………………………………………………………………………（343）

Research progress on lipoprotein metabolism in the hepatitis C virus life cycle YANG Yinrong，WANG Qian……………………………………………………………………………………………………………………（441）

ORIGINAL ARTICLES

Analysis of CYP2C19 gene polymorphism and influence factors of clopidogrel effect in foshan population，Guangdong LIN Jing，YANG Jiefei，LIANG Quanhui，LI Weixuan …………………………………………………………………………………………（6）

Study on single nucleotide polymorphism loci of ORMDL3 gene and susceptibility to asthma in Urumqi Uygur Autonomous State WANG Ling，WEI Yanrong，WANG Lixia，ZHANG Yanli，WANG Jing………………………………………………………………………（11）

The role of miR-34 promoter methylation in the pathogenesis of gastric cancer TIAN Jinghua，YANG Jinling，QIN Jie，ZHAO Linlin，LIU Min，HONG Yanying …………………………………………………………（17）

Relationship between amnesic mild cognitive impairment，MTHFR C677T gene polymorphism and plasma homocysteine level XING Hongyu，LU Ruili，LIANG Ning，ZHOU Junxia…………………………………………………………………………………………（22）

Distribution characteristics of MTHFR and MTRR gene polymorphisms among Han and Li women in Sanya city WU Li，CHEN Shaojin，LI Zhouyang，CHEN qian，LI Yuanli ………………………………………………………………………………（28）

Expression and significance of nuclear factor κB/p65，Bcl-2 and Bax in diffuse large B-cell lymphoma WANG Jing，LI Xinxiao，WU Linna，LIU Fan…………………………………………………………………………………………………（33）

Effects of stress hyperglycemia on inflammatory states，levels of peripheral blood lactate and MMP-9 and 28 d mortality rate in patients with craniocerebral injury WANG Bao，SUN Yuan，SONG Kang，GU Changwei…………………………………………………………………………………………（39）

Predictive value of plasma NT-proBNP level in prognosis of patients with persistent atrial fibrillation after radiofrequency ablation SUN Xiaoyan，XU Liyan，LI Cheng，WU Linna，WEI Lijuan…………………………………………………………………………………（44）

Intervention of human immunoglobulin on liver function，immune function and coagulation function in patients with end-stage liver disease complicated with infection DUAN Hongguang，ZHOU Aijing，CHENG Chongyong，GAO Feng…………………………………………………………………………（49）

Feasibility analysis of irbesartan combined with alprostadil in the treatment of diabetic nephropathy ZHANG Huazhen，ZHAO Xiaoqin，WU Qiongjiao ……………………………………………………………………………………………（54）

The value of serum carcinoembryonic antigen，thyroglobulin and galectin-3 in differential diagnosis of thyroid cancer WEN Huling，XIE Jianping，LIN Shiyu，WEN Dan……………………………………………………………………………………………（58）

Simultaneous detection of deletion and point mutation thalassemia using NGS technology HUANG Jie，YANG Xu，SUN Nan，SUN Binyu，QU Shoufang………………………………………………………………………………（79）

A pedigree analysis of hereditaryⅫdeficiency caused by two missense mutations of p.Glu502Lys and p.Gly542Ser WENG Miaoshan，LIN Fen，ZHANG Jincan，WU Jiaoren，LI Dengsong，XIE Yuqiang，YANG Liye………………………………………（86）

Correlation analysis between PPARGC1 promoter methylation and typeⅡdiabetes LIANG Shuang，WANG Weiwei，SUN Li，ZOU Guanghui，DONG Zhiwu……………………………………………………………………（91）

Correlation between ASAP1 and GSTM3 gene polymorphisms and susceptibility to pulmonary tuberculosis ZHANG Guodong，SONG Gang，LI Minghui …………………………………………………………………………………………………（96）

Investigation on antimicrobial resistance characteristics and mechanism of imipenem-resistant Pseudomonas aeruginosa LI Fei，LI Lijuan，LIANG Dezhi，WANG Fengping，ZHANG Bashan………………………………………………………………………（101）

Study of the distribution of CYP2C19 gene polymorphism in patients with coronary heart disease treated with PCI in Luzhou area PENG Hu，TANG Kebin，YAN Biao，QIU Houbin，ZHU Bin ………………………………………………………………………………（107）

Performance verification and clinical evaluation of HBV DNA high-sensitivity detection kit YU Xuegao，DENG Jiankai，HE Xiaohong，CHEN Peisong，CUI Danli，HUANG Bin……………………………………………………（111）

Effects of L-carnitine combined with hemodialysis on Treg/Th17 cells，serum inflammatory factors and renal function indexes in uremia patients LU Bing，REN Dongsheng，LI Ming…………………………………………………………………………………………………………（117）

Correlation between serum sex hormones，IGF-1，VEGF and tissue LOX levels and lymph node metastasis in patients with breast cancer XING Haixia，ZHAO Mingyan，GUO Yong……………………………………………………………………………………………………（122）

The evaluation of combined detection of serum resistin，VEGF and glycated albumin in early diagnosis of diabetic nephropathy in patients with type 2 diabetes HAN Chenpeng，XU Qingfang，ZHANG Meihua，CAO Liang………………………………………………………………………………（128）

The value of platelet growth factor and interleukin-17 in the diagnosis and prognosis of pulmonary fibrosis XU Lin，LUO Yihua，LI Xia……………………………………………………………………………………………………………………（132）

Effect of metabolic syndrome on prognosis of patients undergoing radical prostatectomy ZENG Mingkun，LIU Ling，ZHANG Xiaomei，MA Tao………………………………………………………………………………………（136）

Analysis of distribution characteristics of high-sensitivity troponin T in the population in Yuxi area，Yunnan YE Dan，FENG Lei，WU Yingsong……………………………………………………………………………………………………………（141）

Preliminary analysis of phenotypic and molecular characterization of CAMP-negative Group B Streptococcus clinical isolate DENG Yingying，KONG Yinbo，JIANG Changhong，DENG Jiankai，JIANG Lingxiao……………………………………………………（164）

Association between acetaldehyde dehydrogenase 2 gene polymorphism and acute myocardial infarction in a southern Chinese Hakka population HOU Jingyuan，XIE Yunquan，ZHAO Pingsen，ZHONG Zhixiong …………………………………………………………………………（170）

Inhibitory effect of lidocaine on the growth of hippocampal neurons in developing period and related mechanisms CHEN Lantao，SUN Xin，YIN Hui，YAO Yafei，DUAN Baomin ……………………………………………………………………………（176）

Detection of drug-resistance gene and homology analysis of Acinetobacter baumannii TAN Pinghai，CHEN Lida，GUO Penghao，LIAO Kang，WU Zhongwen，YE Daning，HUANG Bin ……………………………………（182）

Study on the mechanism of gastric cancer induced by Semaphorin 5A after Helicobacter pylori infection LIU Tao，LI Rui，GONG Lin，LI Geng，PAN Guoqing………………………………………………………………………………………（189）

Expression levels and clinical significance of AKT2 and Annexin A1 in tissues of patients with colon cancer SUN Qingsen，SUN Liansheng，XU Jianli，GU Bin，LI Chao………………………………………………………………………………（194）

Long non-coding RNA LINC00393 in endometrial cancer and its prognostic implications MAI Bi，CHEN Yongxiu，HU Guiying，LUO Xiping，LIU Tingyan………………………………………………………………………… （198）

Analysis of the HPV infection and genotyping distribution in 2 292 women in Fangchenggang city LAN Jiafu………………………………………………………………………………………………………………………………………（204）

Application of circulating tumor cells（CTC）in prognosis and chemotherapy evaluation of breast cancer LIANG Zhihong，CHEN Jiezhen，CHEN Yongxue，GU Wenxin，WU Zehua ………………………………………………………………（209）

Significance of combined detection of HCV RNA and liver function indexes in HCV antibody positive patients ZHU Shaomei，LIU Jihong，ZHOU Xiao………………………………………………………………………………………………………（214）

Expression of LASP-1 Protein in Non-small Cell Lung Cancer and Its Correlation with Clinicopathological Features LI Junqiang，JIANG Maofen，TANG Jingjing，WANG Min，LIU Chunjiao，XIAO Weihua，MA Haifen …………………………………（219）

Effects of pravastatin combined with metoprolol on the cardiac function，glucolipid metabolism and other related indexes in patients with diabetes mellitus complicated with coronary heart disease HAN Lisha，WANG Qiong，LI Dan，WANG Siqi ……………………………………………………………………………………………（224）

Clinical value of Xpert MTB/RIF in detection of Mycobacterium tuberculosis（MTB）and Rifampicin（RIF）resistance WENG Shengfeng，LI Ning，XING Junpeng，ZHANG Changhai，GUO Wei ………………………………………………………………（229）

Establishment and application on the selection and evaluation of automated nucleic acid extraction systems RONG Guodong，ZHAO Hong，WU Lei，HUANG Peijun，WANG Fang，ZHANG Yan，XU Ting…………………………………………（233）

Propofol inhibits the HCT116 cell proliferation and promotes apoptosis of HCT116 cells by regulating the expression of microRNA-133a/SOX4 HAN Jun，ZHAO Jing，ZHANG Liqun…………………………………………………………………………………………………………（238）

Preparation and calibration of the national reference for detection of chromosome copy number variations JIA Zheng，ZHANG Wenxin，LI Lili，SUN Nan，QU Shoufang，HUANG Jie………………………………………………………………（256）

Comparative study on clinical epidemiological characteristics and diagnostic methods of pertussis LI Yue，YUAN Lin，WANG Qing，GAO Wei，SHI Wei，MENG Qinghong，YU Dan，YAO Kaihu…………………………………………（263）

Establishment and application of an antibiotics-resistance gene detection chip for common pathogenic bacteria GUO Jianlian，XIAO Binlong，LIU Huina，JIANG Xianhai，XU Zhongyu…………………………………………………………………（268）

The role of miR-21 in the protective effect of astragaloside on ox-LDL-induced endothelial cell inflammatory injury CHANG Fangyuan，FENG Zerui，XU Yingchun，ZHANG Qiuxia……………………………………………………………………………（276）

miR-548c-3p inhibits cell proliferation，migration and invasion of hepatocellular carcinoma HepG2 cells by targeting TRIM59 NIAN Shiyan，FENG Lei………………………………………………………………………………………………………………………（283）

HD-5 inhibits gastric cancer proliferation，migration and tumor growth SHANG Xiaoyu，GU Guowei，LIU Guoquan，QI Jing，XIE Qiang，LI Kun………………………………………………………………（290）

Effect of cellular immunity combined with operation on quality of life of patients with liver metastasis in colorectal cancer XU Liguo，FENG Guangsen，LIN Yachao，LI Zhi……………………………………………………………………………………………（295）

Solanum nigrum combined with KLF16 gene synergistically inhibit glioma cell proliferation and induce apoptosis ZHAO Shuyang，LEI Yanjie，MA Shijie，GAO Ming…………………………………………………………………………………………（303）

Expression and clinical significances of Galectin-3 mRNA，MUC1 mRNA in peripheral blood of patients with rectal cancer ZHAO Mei，ZHANG Zhihong，GUO Zhongyan，CHEN Ying…………………………………………………………………………………（310）

Effect of Epidural Anesthesia on Cognitive Function and Inflammatory Protein Expression in Patients with Radical Resection of Colon Cancer CHEN Jiehua，MA Haiyan……………………………………………………………………………………………………………………（315）

The changes and clinical significance of serum NT-proBNP，BUA，cTnT levels in patients with uremia before and after hemodialysis XIE Xi，LIU Lili，LI Shuhui……………………………………………………………………………………………………………………（320）

Relationship between the changes of PLT-I，PLT-F，RDW and MCHC indexes and different storage conditions of test specimens HU Yaohua，ZHUO Shuwei，FU Chunmiao…………………………………………………………………………………………………（325）

A surveillance of children lower respiratory tract infection cases in Dongguan City，from 2017 to 2018 SUN Zhihao，ZHANG Ronghua，ZHONG Chaozhen…………………………………………………………………………………………（329）

Establishment and evaluation of a hypersensitivity method of real-time fluorescent PCR for hepatitis B virus detection XIA Qiao，LIAO Lili，DONG Zhiqiang，YANG Honghui，JIANG Xiwen……………………………………………………………………（348）

The application value of high-sensitivity HBV nucleic acid test in preoperative screening LI Baoqi，CHEN Peisong，HUANG Hao，YU Xuegao，HUANG Bin ………………………………………………………………………（355）

Comparative study of high-sensitivity and conventional fluorescence quantitative PCR in the monitoring of antiviral efficacy in patients with chronic hepatitis B LU Jianhua，YANG Li，ZHAO Zhaoxia，LI Qianlin，LI Minran，LIU Yuzhen，DAI Erhei，CHEN Xiuli…………………………………（361）

Clinical value of high sensitive HBV DNA detection in hepatitis B-related hepatocellular carcinoma ZHOU Ying，ZHANG Junxia，HUANG Shu，LIU Liming，GUO Wei………………………………………………………………………（365）

Application of dual-target hyper-sensitivity hepatitis B DNA detection technology in clinical diagnosis of HBeAg negative patients FENG Lei………………………………………………………………………………………………………………………………………（369）

Clinical comparative study of two quantitative methods for HBV DNA detection WANG Shuang，GUO Jie，WANG Dagang，SHI Jingren，PAN Meichen，YIN Shangqi，HE Chaonan，MENG Huan，ZHANG Xiang，WANG Yajie……………………………………………………………………………………………………………………（374）

The clinical application of high sensitivity HCV RNA detection for diagnosis and treatment in patients with chronic hepatitis C HUANG Chengjun，CHENG Shuquan…………………………………………………………………………………………………………（379）

Clinical comparative study of two methods for quantitative detection of HCV RNA PENG Yabai，PENG Yalan，GUAN Jipeng，WANG Min ……………………………………………………………………………………（383）

Clinical characteristics of chronic hepatitis B patients with both HBeAg and HBeAb positive LUO Lin，ZHANG Tingchao，LIU Shugang……………………………………………………………………………………………………（387）

Clinical significance of cystatin C and hepatic artery resistance index in patients with liver cirrhosis SHAO Xiaomei，YANG Yang，HAN Lixia………………………………………………………………………………………………………（391）

The clinical effect and immune function of anti HBV on recurrence and metastasis of liver cancer after transarterial chemoembolization CHEN Ming，CAO Yang………………………………………………………………………………………………………………………（396）

miR-3182 promotes the apoptosis of hepato-carcinoma cells and enhance the radio-sensitivity of liver cancer cells YU Fengqin，XU Yunfang，ZHANG Qing………………………………………………………………………………………………………（401）

Study on the value of peripheral blood cytokines in the treatment of chronic hepatitis B SUI Jia，YU Meng，ZHU Shuzhen，WEI Qinzheng……………………………………………………………………………………………（408）

Correlation analysis of HCV RNA，HCV-cAg，SOD and FFA in hepatitis C patients FANG Weizhen，GU Wenshen，FANG Qimei，ZENG Wuyi，DING Rui，DUAN Chaohui…………………………………………………（414）

Study on miR-122 and miR-150 in differential diagnosis of occult hepatitis MA Qingsong，ZHANG Yunfen…………………………………………………………………………………………………………………（418）

Establishment and evaluation of next-generation sequencing for the detection of hepatitis B virus YMDD-motif mutations YU Xuegao，DENG Jiankai，CHEN Yaoming，CHEN Peisong，HE Xiaohong，ZHONG Liangying，HUANG Bin ………………………（446）

Correlation between methylation of snail gene and EMT induced by TGFβ1 in prostate cancer cells DU Yandan，NIU Yiqing，ZHENG Haijun，WANG Xiaoyan，JIANG Yuhai，CHEN Haiqiu，LI Yinyan，SUN Gang，LI Chunyu，LU Desheng，SUN Hui……………………………………………………………………………………………………………（451）

Evaluation of serum HMGB1，TGF-β1 and GP73 in the prognosis of sepsis complicated with liver injury SONG Ying，LI Shipeng，JIAO Weiwei ………………………………………………………………………………………………………（457）

Effect of miR-137 targeting down-regulation of SETD7 expression on oxidative stress induced by hypoxia-reoxygenation in cardiomyocytes WANGYanli，LI Jiming，LUO Jinguang………………………………………………………………………………………………………（462）

Analysis of driving gene mutations in non-small cell lung cancer using UMI-based next-generation sequencing LIU Xiaoyun，WU Xiaoyan，SHAO Qiong，LONG Yakang，WANG Haiyun，DENG Ling…………………………………………………（468）

Study on the single nucleotide polymorphisms of PSCA gene and susceptibility to gastric ulcer MO Dachao，LI Junjiu，PENG Liang，LIU Zhiyuan，WANG Jieyun，YUAN Jiru …………………………………………………………（474）

Study on the effect of FBN1 gene c.2418A＞G mutation on transcription ZHANG Yue，LI Jinjie，DIAO Yanjun，YANG Liu，HAO Xiaoke……………………………………………………………………………（479）

Effect of lentivirus mediated HMGN5 gene silencing on proliferation，migration and invasion of ovarian cancer cells and its mechanism SUN Juan，XIN Yanping，ZHANG Guomei，TIAN Xiaona，LIU Huimin……………………………………………………………………（485）

Predictive value of early detection of PTX-3 and PCT for pulmonary infection after gynecological laparoscopic surgery under general anesthesia YANG Bingrong，WANG Jin……………………………………………………………………………………………………………………（491）

Serum HBV-DNA，preS1，HBeAg in patients with various HBV genotype of chronic virus hepatitis B WU Pai，WU Kunhe，LIU Haiyan，ZHANG Douxing，HU Anqun…………………………………………………………………………（495）

Chromosome karyotype analysis of 1 776 peripheral blood lymphocytes in Nanjing Area YU Mengyao，GU Min，ZHAO Hong …………………………………………………………………………………………………………（499）

The diagnostic value of serum chemerin，25（OH）D3，visfatin levels in gestational diabetes mellitus and their association with insulin resistance LI Maoxiang，LI Yu，LYU Yuanyuan，CAO Yongxian………………………………………………………………………………………（503）

Correlation between CEA，NSE，SCCA combined with miR-21 and clinicopathological features，efficacy and prognosis in patients with lung cancer TANG Qiaoya，GONG Junming，MA Yanling…………………………………………………………………………………………………（508）

Changes and significance of serum miR-21 and carbohydrate antigen 125 in patients with ovarian cancer YOU Juan………………………………………………………………………………………………………………………………………… (513)

Effect of cleistocalyx operculatus flower buds on hydrogen peroxide induced apoptosis of nerve cells YU Guangzhou，TAN Wengang，ZHANG Hao………………………………………………………………………………………………… (517)

The clinical significance of plasma mir-192 and mir-29c levels in diagnosis of type 2 diabetic nephropathy HE Shuang，HUANG Ping，FAN Mingjuan…………………………………………………………………………………………………… (522)

Expression of interleukin-6 and procalcitonin in serum of patients with sepsis and its clinical significance LIU Leilei，HUA Wei，JU Peihong，ZHANG Wenyan，HUANG Kan，LI Zheng …………………………………………………………… (527)

Diagnostic value of D-lactic acid and procalcitonin in bacterial meningitis FAN Wenbing，ZHOU Yinsuo…………………………………………………………………………………………………………………… (531)

Expression and significance of GATA3 and CK20 in bladder urothelial carcinoma ZENG Yumei，DU Juan，CHEN Yingzhi，CHU Bin…………………………………………………………………………………………… (535)

REVIEWS

Regulation of miRNAs on the growth，development and function of natural killer cells HUANG Qiuping，YUAN Huan，WANG Yilin，HUANG Qingsheng，LI Qi……………………………………………………………………（63）

Progress in screening and diagnostic techniques for α-thalassemia SHEN Ru，CHEN Yunming，YANG Xiaohong，WANG Xiaoyan………………………………………………………………………………（68）

Advances in the application of imaging and molecular detection technology in the diagnosis of Alzheimer's disease LI Zhuoran，WEI Xinhua，JIANG Xinqing……………………………………………………………………………………………………（146）

Application of Next-Generation Sequencing in the Diagnosis of Genetic related Intellectual Disability SHEN Ru，REN Baoyan，JIANG Hongchao，WANG Xiaoyan ………………………………………………………………………………（152）

Research progress of dendritic cells and neutrophils in the pathogenesis of systemic lupus erythematosus QING Pingying，LIU Yi………………………………………………………………………………………………………………………（245）

Advances in genetic testing of spontaneous abortion LIU Xiangju……………………………………………………………………………………………………………………………………（338）

Current status，questions and trend of the clinical ultrasensitive hepatitis B virus DNA detection LI Jin，LI Yirong………………………………………………………………………………………………………………………………（422）

Clinical application of hypersensitive HCV RNA detection technology LIU Na，LI Chunxia，DONG Bing，ZHOU Lulu，ZHANG Ruiqin，XU Guanghua…………………………………………………………（428）

Progress in research on hepatitis B virus resistance ZHANG Jinfeng，AN Hongliang，SU Rong，LV Weifeng……………………………………………………………………………………（434）

Progress in clinical application of circulating tumor DNA CHENG Xiaowen………………………………………………………………………………………………………………………………（539）