HO-1 up-regulation decreases liver ischemia/reperfusion injury by inhibiting mast cells degranulation

/Tao Shaofu

(陶绍富，Dept Gen Surg，Xinhua Hosp，Shanghai Jiangtong Univ，School Med，Shanghai 200092)…∥Chin J Gen Surg.-2011，26(9).-766～769

ObjectiveTo investigate the effects of HO-1 on mast cells degranulation during liver ischemia/reperfusion injury.MethodsSD rats(n=20)were randomly divided into 4 groups:(1)sham operation group，(2)ischemia/reperfusion injury group(I/RI group)，(3)CoPP group，CoPP was given before 24 h(5 mg/kg)，(4)ZnPP group，ZnPP was given before 24 h(20 mg/kg).The rat model of liver ischemia/reperfusion was built.Samples were collected after 2 hours reperfusion.HO-1 mRNA expression was assessed by RT-PCR.The expression of HO-1 protein was assessed by Western blot.Serum ALT，AST levels were determined.The amount of degranulated mast cells were detected by toluid-ine blue staining.Liver injuries were evaluated by HE staining.ResultsCompared to sham group，liver HO-1mRNA and protein expression，ALT，AST levels，the number of degranulated mast cells significantly increased in I/RI group，CoPP group and ZnPP group.Moreover，liver injuries were more serious.Compared to I/RI group，there were increased HO-1mRNA and protein expression，reduced ALT，AST levels and the number of degranulated mast cells，also reduced liver injuries were found in CoPP group.Compared to I/RI group，the expression of HO-ImRNA and protein was down-regulated，ALT，AST levels elevated and the number of degranulated mast cells increased accompanying more serious liver injuries in ZnPP group.ConclusionHO-1 overexpression reduces liver ischemia/reperfusion injury，possibly by inhibiting mast cells degranulation in liver tissues.11 refs.5 figs ，1 tab