The VEGF production by dedifferentiated chondrocytes under synovial fluid stimulation from coxarthrosis and femoral neck fracture patients

于腾波(Yu Tengbo，Dept Orthop，Affil Hosp Med Col，Qingdao Univ，Qingdao 266003)…∥Chin J Orthop.－ 2010，30(12).－1206～1210

The VEGF production by dedifferentiated chondrocytes under synovial fluid stimulation from coxarthrosis and femoral neck fracture patients

于腾波(Yu Tengbo，Dept Orthop，Affil Hosp Med Col，Qingdao Univ，Qingdao 266003)…∥Chin J Orthop.－ 2010，30(12).－1206～1210

ObjectiveTo investigate the vascular endothelial growth factor(VEGF)expression level by chondrocytes isolated from patients with osteoarthritis(OA)in hip or femoral neck fracture(FNF)and explore the effect of synovial fluid from OA or FNF on secretion of VEGF.MethodsThe cartilage tissues were collected from 12 patients with OA in hip and 8 patients with FNF.Cartilage was stained with HIM and Safranin O/Fast Green(S/F)method.The damage of cartilage was evaluated using Mankin scores.Cathepsin B which was selected for cell dedifferentiation monitoring marker and VEGF level was detected in the supernatant fluid.The synovial fluid from OA，FNF and DMEM were respectively added to the culture medium to explore their effects on regulating VEGF.ResultsFor cartilage，the Mankin scores of OA group were higher than that of FNF group.Chondrocytes gradually lost their original spherical appearance，with Cathepsin B upregulated while VEGF downregulated.The OA synovial fluid can stimulate chongdrocytes to secrete more VEGF than that from patients with FNF.However，chondrocytes gradually produced less VEGF after passaging.ConclusionMankin scores had good correlation with chondrocytes’VEGF production in the early stage of primary culture.Chondrocytes showed quick dedifferentiation characteristicsin vitro.OA synovialfluid showed a stronger capability in stimulating chondrocytes to express more VEGF，which might indicate that OA synovial fluid participated in the pathological process of OA.18 refs，4 tabs.

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