Expression of transforming growth factor-β in local bony callus in traumatic brain injury combined with extremity fracture in rats

张胜利(Zhang Shengli，Dept Traum Orthop，2nd Peop Hosp Shenzhen，Shenzhen 518035)…∥Chin J Orthop Trauma.－2011，13(2).－175～177

Expression of transforming growth factor-β in local bony callus in traumatic brain injury combined with extremity fracture in rats

张胜利(Zhang Shengli，Dept Traum Orthop，2nd Peop Hosp Shenzhen，Shenzhen 518035)…∥Chin J Orthop Trauma.－2011，13(2).－175～177

ObjectiveTo investigate gene expression of transforming growth factor-β(TGF-β)in local bony callus in tracumatic brain injury combined with extremity long bone fracture in rats.MethodsEighty male SD rats were randomized into 2 even groups:the experimental group were models of traumatic brain injury combined with extremity bone fracture and the control group were models of simple extremity bone fracture.Samples of bony callus were harvested at 1，2，3，and 4 weeks in both groups，each time from 10 rats respectively，for detection of gene expressions of TGF-β by reverse transcription PCR techniques.ResultsLevels of gene expression of TGF-β in local bony callus in the experimental group were significantly higher than those in the control group at one week(P ＜ 0.05);but significantly lower at 3 weeks(P ＜ 0.05).Peak values occurred at 2 week in both groups，though，significantly higher than those at other times，there was no significant difference between the 2 groups(P ＞0.05).ConclusionChanges in gene expression of TGF-β in local bony callus in traumatic brain injury combined with extremity long bone fracture indicate that TGF-β may play a role in the process of increased fracture healing.13 refs，1 tab.

(Authors)